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Corneal regenerative medicine : methods and protocols

Author: Bernice Wright; Che J Connon; Springer Science+Business Media,
Publisher: New York : Humana Press, Springer Science+Business Media, [2013] ©2013
Series: Methods in molecular biology (Clifton, N.J.), v. 1014.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of  Read more...
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Genre/Form: Laboratory Manuals
Laboratory manuals
Document Type: Book
All Authors / Contributors: Bernice Wright; Che J Connon; Springer Science+Business Media,
ISBN: 9781627034319 1627034315
OCLC Number: 828892142
Description: xii, 230 pages : illustrations (some color) ; 26 cm.
Contents: Limbal epithelial cell therapy : past, present, and future / Tor Paaske Utheim --
Artificial cornea / Naresh Polisetti, Mohammad Mirazul Islam, and May Griffith --
Clonal analysis of limbal epithelial stem cell populations / Ursula Schlötzer-Schrehardt --
Use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation / Ashley M. Crane and Sanjoy K. Bhattacharya --
Enrichment of human corneal epithelial stem/progenitor cells by magnetic bead sorting using SSEA4 as a negative marker / Martin N. Nakatsu and Sophie X. Deng --
Limbal epithelial stem cell identification using immunoblotting analysis / Bernice Wright and Che J. Connon --
Culture of limbal epithelial cells / Tor Paaske Utheim, Torstein Lyberg, and Sten Ræder --
Culture of limbal stromal cells and corneal endothelial cells / Naresh Polisetti and Nancy C. Joyce --
Formation of a tissue-engineered cornea using plastically compressed collagen scaffolds and limbal stem cells / Shengli Mi and Che J. Connon --
Fabrication of a human recombinant collagen-based corneal substitute using carbodiimide chemistry / Mohammad Mirazul Islam, May Griffith, and Kimberley Merrett --
Fabrication of a corneal-limbal tissue substitute using silk fibroin / Laura J. Bray [and 4 others] --
Cultivation of limbal epithelial cells on electrospun poly(lactide-co-glycolide) scaffolds for delivery to the cornea / Pallavi Deshpande [and 3 others] --
Growth and delivery of mesenchymal and limbal stem cells using copolymer polyamide 6/12 nanofiber scaffolds / Vladimir Holan, Eliska Javorkova, and Peter Trosan --
Encapsulation and culture of mammalian cells including corneal cells in alginate hydrogels / Nicola C. Hunt and Liam M. Grover --
In vivo confocal microscopy of the cornea to assess tissue regenerative response after biomaterial implantation in humans / Neil Lagali, May Griffith, and Per Fagerholm.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1014.; Springer protocols (Series)
Responsibility: edited by Bernice Wright and Che J. Connon.

Abstract:

This book provides a concise overview of essential techniques in corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine.  Read more...

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Primary Entity

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