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Cryopreservation and freeze-drying protocols

Author: John G Day; Mark R McLellan
Publisher: Totowa, N.J. : Humana Press, ©1995.
Series: Methods in molecular biology (Clifton, N.J.), volume 38.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials. Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or  Read more...
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Genre/Form: Fulltext
Internet Resource
Electronic books
Additional Physical Format: Print version:
Cryopreservation and freeze-drying protocols.
Totowa, N.J. : Humana Press, 1995
(DLC) 94044732
(OCoLC)31753687
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: John G Day; Mark R McLellan
ISBN: 9781592595259 1592595251
OCLC Number: 230742924
Language Note: English.
Description: 1 online resource (x, 254 pages) : illustrations.
Contents: Virus cryopreservation and storage / Ernest A. Gould --
Freeze-drying and cryopreservation of bacteria / Stephen F. Perry --
Freeze-drying of yeasts / Sugio Kawamura, Yukie Murakami, Yukie Miyamoto, Kazuo Kimura --
Cryopreservation of yeast cultures / Chris J. Bond --
Cryopreservation and freeze-drying of fungi / Jacqueline A. Kolkowski, David Smith --
Cryopreservation of pathogenic and nonpathogenic free-living amoebae / Simon Kilvington --
Long-term cryopreservation of thylakoid membranes / Dirk K. Hincha, Jürgen M. Schmitt --
Cryopreservation of algae / John G. Day, Mitzi M. DeVille --
Cryopreservation of plant protoplasts / Brian W.W. Grout --
A two-step or equilibrium freezing procedure for the cryopreservation of plant cell suspensions / Elly W. Schrcnemakers, Frank Van Iren --
Vitrification of plant cell suspensions / Poula J. Reinhoud, Atsuko Uragami, Akira Sakai, Frank van Iren --
Cryopreservation of shoot-tips and meristems / Erica E. Benson --
Cryopreservation of seeds / Hugh W. Pritchard --
Cryopreservation of the sperm of the Pacific oyster Crassostrea gigas / Iain R. McFadzen --
Cryopreservation of fish spermatozoa / Krishen J. Rana --
Cryopreservation of avian spermatozoa / Graham J. Wishart --
Cryopreservation of animal and human cell lines / Christopher B. Morris --
Cryopreservation of semen from domestic livestock / Mark R. Curry --
Cryopreservation of mammalian embryos. Slow cooling / Jocelyn E. Hunter --
Cryopreservation of mammalian embryos. Vitrification / Magosaburo Kasai --
Cryopreservation of human gametes / Jocelyn E. Hunter --
Cryopreservation of human red blood cells / Michael J. Thomas, Susan H. Bell.
Series Title: Methods in molecular biology (Clifton, N.J.), volume 38.
Responsibility: edited by John G. Day and Mark R. McLellan.
More information:

Abstract:

This book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials. Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or tissue type, and is accessible even to the nonspecialist because of its cookbook approach. Novel protocols can be readily developed with the help of the "hands-on" Notes sections. Cryopreservation and Freeze-Drying Protocols is an indispensable reference work for both the individual researcher, and all those who want to establish or improve biostorage systems in their laboratories. Its applications range from microbial culture collections, botanic gardens, and zoos to animal husbandry, aquaculture, medicine, human fertilization, and cell and molecular biology.

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