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Crystallographic methods and protocols

Author: Christopher Jones; Barbara Mulloy; Mark R Sanderson
Publisher: Totowa, N.J. : Humana Press, ©1996.
Series: Methods in molecular biology (Clifton, N.J.), v. 56.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
"In Crystallographic Methods and Protocols leading specialists in the application of X-ray crystallography to biological problems provide a basic and comprehensive guide to its successful use in obtaining detailed three-dimensional structures of proteins, nucleic acids, and their complexes. These deeply experienced researches describe in clear practical terms their proven methods for preparing samples, crystallizing  Read more...
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Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Christopher Jones; Barbara Mulloy; Mark R Sanderson
ISBN: 0896032590 9780896032590
OCLC Number: 34246158
Description: xii, 394 pages : illustrations ; 23 cm.
Contents: Overexpression, isolation, and crystallization of proteins / Jane V. Skelly, C. Bernadette Madden --
Preliminary characterization of crystals / Sherin S. Abdel-Meguid, David Jeruzalmi, Mark R. Sanderson --
Modern methods for rapid X-Ray diffraction data collection from crystals of macromolecules / Elspeth F. Garman --
Use of multiple-wavelength anomalous diffraction measurements in ab initio phase determination for macromolecular structures / H.M. Murthy --
Structure determination using isomorphous replacement / Sherin S. Abdel-Meguid --
Molecular replacement using known structural information / Ian J. Tickle, Huub P. Driessen --
Density modification in X-ray crystallography / Alberto D. Podjarny, Bernard Rees, Alexandre G. Urzhumtsev --
Refinement of protein and nucleic acid structures / Eric Westhof, Philippe Dumas --
Recent developments for crystallographic refinement of macromolecules / Axel T. Brünger --
The crystallization and structure analysis of oligonucleotide sequences / Stephen Neidle --
Crystallography in the study of protein-DNA interactions / David G. Brown, Paul S. Freemont --
Virus crystallography / Elizabeth Fry, Derek Logan, David Stuart --
Crystallization and structure analysis of membrane proteins / Richard Newman.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 56.
Responsibility: edited by Christopher Jones, Barbara Mulloy, and Mark R. Sanderson.

Abstract:

"In Crystallographic Methods and Protocols leading specialists in the application of X-ray crystallography to biological problems provide a basic and comprehensive guide to its successful use in obtaining detailed three-dimensional structures of proteins, nucleic acids, and their complexes. These deeply experienced researches describe in clear practical terms their proven methods for preparing samples, crystallizing and characterizing them, acquiring and analyzing data, and solving and refining structures. They then share how to apply these state-of-the-art techniques to nucleic acids, proteins, viruses, and membrane proteins, as well as to elucidating the structure of such macromolecules as synthetic nucleic acid oligomers and protein-nucleic acid complexes."--BOOK JACKET. "Crystallographic Methods and Protocols offers rich insights into and broad understanding of the strengths and limitations of X-ray crystallography. It gives biologically trained workers ready access to a powerful technique capable of helping them to solve many of today's biologically relevant problems."--BOOK JACKET.

Table of Contents:

by umamaheswarreddy (WorldCat user on 2007-01-05)

Table of Contents Introduction. Overexpression, Isolation, and Crystallization of Proteins. Preliminary Characterization of Crystals. Modern Methods for Rapid X-Ray Diffraction Data Collection from Crystals of Macromolecules. Use of Multiple-Wavelength Anomalous Diffraction Measurements in Ab Initio Phase Determination for Macromolecular Structures. Structure Determination Using Isomorphous Replacement. Molecular Replacement Using Known Structural Data. Density Modification in X-Ray Crystallography. Refinement of Protein and Nucleic Acid Structures. Recent Developments for Crystallographic Refinement of Macromolecules. The Crystallization and Structure Analysis of Oligonucleotide Sequences. Crystallography in the Study of Protein–DNA Interactions. Virus Crystallography. Crystallization and Structure Analysis of Membrane Proteins.

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