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Cytochrome P450 protocols

Author: Ian R Phillips; Elizabeth A Shephard
Publisher: Totowa, N.J. : Humana Press, ©1998.
Series: Methods in molecular biology (Clifton, N.J.), v. 107.
Edition/Format:   Print book : EnglishView all editions and formats
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Genre/Form: Laboratory manuals
Manuels de laboratoire
Additional Physical Format: Online version:
Cytochrome P450 protocols.
Totowa, N.J. : Humana Press, ©1998
(OCoLC)607100557
Online version:
Cytochrome P450 protocols.
Totowa, N.J. : Humana Press, ©1998
(OCoLC)608572617
Document Type: Book
All Authors / Contributors: Ian R Phillips; Elizabeth A Shephard
ISBN: 0896035190 9780896035195
OCLC Number: 38324391
Description: xiv, 482 pages : illustrations ; 24 cm.
Contents: The role of methods in the discovery of the cytochromes P450 / Ronald W. Estabrook --
Cytochrome P450 Nomenclature / David R. Nelson --
Spectral analyses of cytochromes P450 / John B. Schenkman, Ingela Jansson --
Purification of cytochromes P450 : rat and human hepatic forms / F. Peter Guengerich, Martha V. Martin --
Isolation and purification of constitutive forms of microsomal cytochrome P450 / John B. Schenkman, Ingela Jansson --
Purification of extrahepatic cytochromes P450 / Margaret Warner, Jan-Ake Gustafsson --
Purification of cytochromes P450 : products of bacterial recombinant expression systems / F. Peter Guengerich, Natalie A. Hosea, Martha V. Martin --
Cytochrome P450 reconstitution systems / Tsutomu Shimada, Hiroshi Yamazaki --
Catalytic assays for human cytochrome P450 / Thomas K.H. Chang, David J. Waxman --
Enzymatic analsis of cDNA-expressed human CYP1A1, CYP1A2, and CYP1B1 with 7-ethoxyresorufin as substrate / Thomas K.H. Chang, David J. Waxman --
Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydrolation / David J. Waxman, Thomas K.H. Chang --
Determination of the CYP2B6 component of 7-ethoxy-4 trifluoromethycloumarin O-deethylation activity in human liver microsomes / Thomas K.H. Chang, SCharles L. Crespi, David J. Waxman --
High-performance liquid chromatographic analysis of CYP2C8-catalyzed paclitaxel 6[alpha]-hydroxylation / Charles L. Crespi, Thomas K.H. Chang, David J. Waxman --
Determination of CYP2C9-catalyzed diclofenac 4'-hydroxylation by high-performance liquid chromatography / Charles L. Crespi, Thomas K.H. Chang, David J. Waxman --
CYP2C19-mediated (S)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromatography with radiometric detection / Charles L. Crespi, Thomas K.H. Chang, David J. Waxman --
CYP2D6-dependent bufuralol 1'-hydroxylation assayed by reversed-phase ion-pair high-performance liquid chromatography with fluorescence detection / Charles L. Crespi, Thomas K.H. Chang, David J. Waxman --
Spectrophotometric analysis of human CYP2E1-catalyzed [rho]-nitrophenol hydroxylation / Thomas K.H. Chang, Charles L. Crespi, David J. Wacman --
Thin-layer chromatographic analysis of human CYP3A-catalyzed testosterone 6[beta]-hydroxylation / David J. Waxman, Thomas K.H. Chang --
Determination of CYP4A11-catalyzed lauric acid 12-hydroxylation by high-performance liquid chromatography with radiometric detection / Charles L. Crespi, Thomas K.H. Chang, David J. Waxman --
An isocratic high-performance liquid chromatographic assay for CYP7A1-catalyzed cholesterol 7[alpha]-hydroxylation / David J. Waxman, Thomas K.H. Chang --
Use of 7-ethoxycoumarin to monitor multiple enzymes in the CYP1, CYP2, and CYP3 families / David J. Waxman, Thomas K.H. Chang --
Expression of eukaryotic cytochromes P450 in E. coli / Christopher M. Jenkins, Irina Pikuleva, Michael R. Waterman --
Expression of mammalian cytochromes P450 in yeast / Yoshiyasu Yabusaki --
Expression of cytochromes P450 in a baculovirus system / Stephen R. Hood, Girish Shah, Peter Jones --
Expression of cytochromes P450 in mammalian cells / Els M. de Groene --
Monoclonal antibodies to cytochromes P450 / Harry V. Gelboin [and others] --
Targeting antipeptide antibodies towards cytochrome P450 enzymes / Robert J. Edwards --
Signals for retention of cytochrome P450 in the endoplasmic reticulum / Elizbieta Skorupa, Byron Kemper --
Quantification of cytochrome P450 gene expression by RNase protection analysis / Colin N.A. Palmer --
Hepatocyte cultures in drug metabolism and toxicological research and testing / Vera Rogiers, Antoine Vercruysse --
Isolation of rat hepatocytes / Karen De Smet [and others] --
Collagen-gel cultures of rat hepatocytes : collagen-gel sandwich and immobilization cultures / Sonja Beken [and others] --
Rat hepatocyte cultures : conventional monolayer cultures and cocultures with rat liver epithelial cells / Tamara Vanhaecke [and others] --
Isolation of periportal and pericentral hepatocytes / Rolf Gebhardt --
Perifusion culture of hepatocytes / Rolf Gebhardt --
Human hepatocyte culture / Jean Bernard Ferrini [and others] --
Reconstruction of human epidermis in vitro / Marie-Cecile Lenoir-Viale --
Gene transfer and expression / Frank Gaunitz, Monika Papke --
Lipid-mediated gene transfer into normal adult human hepatocytes in primary culture / Jean-Claude Ourlin [and others] --
Identification of regulatory protein-binding sites in cytochrome P450 genes by means of the gel-retardation assay / Helen Dell [and others] --
Cytochrome P450 gene regulation : analysis of protein-DNA interactions in situ / Steven T. Okino, James P. Whitlock --
Analysis of cytochrome P450 polymorphisms / Ann K. Daly [and others] --
Induction of cytochrome P450 1A (CYP1A) in fish : a biomarker for environmental pollution / Bente M. Nilsen, Karin Berg, Anders Goksoyr --
Generation allelic series in mice by gene targeting / Maureen K. Bunger, Christopher A. Bradfield.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 107.
Other Titles: Cytochrome P four hundred fifty protocols
Responsibility: edited by Ian R. Phillips and Elizabeth A. Shephard.

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