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Dengue : methods and protocols

Author: Radhakrishnan Padmanabhan; Subhash Vasudevan
Publisher: New York, NY : Humana Press, [2014] ©2014
Series: Methods in molecular biology (Clifton, N.J.), v. 1138.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue:  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Dengue.
New York, NY : Humana Press, [2014]
(DLC) 2014933052
(OCoLC)881453825
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Radhakrishnan Padmanabhan; Subhash Vasudevan
ISBN: 9781493903481 1493903489 1493903470 9781493903474
OCLC Number: 877823584
Description: 1 online resource (xiii, 403 pages) : illustrations (some color).
Contents: Dengue Virus Growth, Purification and Fluorescent Labeling --
Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique --
Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique --
Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy --
Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion --
Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection --
Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging --
Development and Application of Dengue Virus Reverse Genetic Systems --
DENV4 Renilla Luciferase Reporter Replicons --
Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination --
Identification of Dengue-Specific Human Antibody Fragments Using Phage Display --
Next Generation Whole Genome Sequencing of Dengue Virus --
MPGAfold in Dengue Secondary Structure Prediction --
Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE --
Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 --
Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry --
Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore --
Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation --
Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import --
Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening --
Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors --
Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase --
Animal Models in Dengue --
Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1138.
Responsibility: edited by Radhakrishnan Padmanabhan, Subhash G. Vasudevan.

Abstract:

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact  Read more...

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