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Determination of the inhibition of the activity of anaerobic bacteria : reduction of gas production from anaerobically digesting (sewage) sludge

Author: Organisation for Economic Co-operation and Development.
Publisher: [Paris] : OECD Publishing, 2007.
Series: OECD Guidelines for the Testing of Chemicals., Section 2,, Effects on Biotic Systems.
Edition/Format:   eBook : Document : International government publication : EnglishView all editions and formats
Summary:
This Test Guideline describes a method, which can provide information that is useful in predicting the likely effect of a test substance on gas production in anaerobic digesters. Aliquots of a mixture of anaerobically digesting sludge (20 g/L to 40 g/L total solids) and a degradable substrate solution are incubated alone and simultaneously with a range of concentrations of the test substance in sealed vessels for up  Read more...
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Material Type: Document, Government publication, International government publication, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Organisation for Economic Co-operation and Development.
ISBN: 9789264067332 9264067337
OCLC Number: 704445360
Description: 1 online resource (19 pages).
Series Title: OECD Guidelines for the Testing of Chemicals., Section 2,, Effects on Biotic Systems.
Other Titles: 224, adopted 8 January 2007
Responsibility: Organisation for Economic Co-operation and Development.

Abstract:

This Test Guideline describes a method, which can provide information that is useful in predicting the likely effect of a test substance on gas production in anaerobic digesters. Aliquots of a mixture of anaerobically digesting sludge (20 g/L to 40 g/L total solids) and a degradable substrate solution are incubated alone and simultaneously with a range of concentrations of the test substance in sealed vessels for up to 3 days. At least triplicate sets of bottles for each of a range of concentrations are used. This study includes measurement of pressure (indication about the amount of gas) and pH. The percentage inhibition of gas production brought about by the various concentrations of the test substance is calculated from the amounts produced in the respective test and control bottles (with 3,5-dichlorophenol). The EC50 and other effective concentrations are calculated from plots of percentage inhibition against the concentration of the test chemicals or, more usually, its logarithm.

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