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Determining mycotoxins and mycotoxigenic fungi in food and feed

Author: Sarah De Saeger
Publisher: Cambridge, UK ; Philadelphia, PA : Woodhead Publishing, 2011.
Series: Woodhead Publishing in food science, technology, and nutrition, no. 203.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Electronic books
Field guides
Additional Physical Format: Print version:
Determining mycotoxins and mycotoxigenic fungi in food and feed
(OCoLC)666239739
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Sarah De Saeger
ISBN: 9780857090973 0857090976
OCLC Number: 867050267
Description: 1 online resource (xxiv, 427 pages) : illustrations.
Contents: Cover; Determining mycotoxinsand mycotoxigenic fungiin food and feed; Copyright; Contents; Contributor contact details; Woodhead Publishing Series in Food Science, Technology and Nutrition; Preface; Part I Determining mycotoxins in food and feed; 1 Sampling strategies to control mycotoxins; 1.1 Food safety and the requirements for international food trade; 1.2 Principles of food and feed sampling for mycotoxin analysis; 1.3 International guidance on sampling food and feed for mycotoxin analysis. 1.4 Uncertainty estimation and designing sound sampling plans for mycotoxin analysis in food and feed1.5 Quality assurance and quality control procedures in sampling and arrival of the samples at the analytical laboratory; 1.6 Strengthening national food control systems; 1.7 Sources of further information and advice; 1.8 References; 2 Sample preparation and clean up in mycotoxin analysis: principles, applications and recent developments; 2.1 Introduction; 2.2 Methods used for extraction and clean up of mycotoxins from complex matrices; 2.3 Recent developments; 2.4 Conclusions. 2.5 Acknowledgements2.6 References; 3 Chromatographic separation techniques for determination of mycotoxins in food and feed; 3.1 Introduction; 3.2 Thin-layer chromatography in mycotoxin analysis of food and feed; 3.3 Gas chromatography in mycotoxin analysis of food and feed; 3.4 High-performance liquid chromatography in mycotoxin analysis of food and feed; 3.5 Electrophoretic separations in mycotoxin analysis of food and feed; 3.6 Future trends; 3.7 Sources of further information and advice; 3.8 References; 4 Mass spectrometry in multi-mycotoxin and fungal spore analysis; 4.1 Introduction. 4.2 Liquid chromatography-mass spectrometry (LC-MS) methods in multi-mycotoxin analysis4.3 Liquid chromatographic aspects of multi-mycotoxin methods; 4.4 Mass spectrometric aspects of multi-mycotoxin methods; 4.5 LC-MS aspects of multi-mycotoxin analysis; 4.6 Future trends in liquid chromatography-mass spectrometry analysis; 4.7 Conclusions; 4.8 Acknowledgements; 4.9 References; 5 Immunochemical methods for rapid mycotoxin detection in food and feed; 5.1 Introduction; 5.2 Antibody production and characterization. 5.3 Specificity of immunochemical methods for rapid mycotoxin detection in food and feed5.4 Microtiter plate enzyme-linked immunosorbent assay for rapid mycotoxin detection in food and feed; 5.5 Non-instrumental rapid tests for mycotoxin detection in food and feed; 5.6 Conclusions and future trends; 5.7 References; Part II Quality assurance and official methods for determining mycotoxins in food and feed; 6 Official methods and performance criteria for determining mycotoxins in food and feed; 6.1 Introduction; 6.2 Official control laboratories for determining mycotoxins in food and feed.
Series Title: Woodhead Publishing in food science, technology, and nutrition, no. 203.
Responsibility: edited by Sarah De Saeger.

Abstract:

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Mycotoxins - toxic secondary metabolites produced by mycotoxigenic fungi pose a significant risk to the food chain. Indeed, they may be the most hazardous of all food contaminants in terms of chronic toxicity and legislative limits on their levels in food and feed continue to be developed worldwide. Rapid and reliable methods for the determination of both mycotoxigenic fungi and mycotoxins in food and feed are therefore essential. This book reviews current and emerging methods in this area. Part one focuses on the essentials of mycotoxin determination, covering sampling, sample preparation and clean-up and key determination techniques, such as chromatographic separation, liquid chromatography-mass spectrometry and immunochemical methods. Part two then goes on to describe quality assurance, official methods and performance criteria for determining mycotoxins in food and feed. Topics covered include laboratory accreditation, method validation and measurement uncertainty. The development and analysis of biomarkers for mycotoxins are discussed in part three. Individual chapters focus on detecting exposure in humans and animals. Part four is concerned with the processes involved in determining mycotoxigenic fungi in food and feed. It also describes the identification of genes and gene clusters involved in mycotoxin synthesis, as well as DNA barcoding of toxigenic fungi. Finally, Part five explores some of the emerging methods for mycotoxin analysis, ranging from bio-sensing to spectroscopic techniques.

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