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Dictyostelium discoideum protocols

Author: Ludwig Eichinger; Francisco Rivero-Crespo
Publisher: New York : Humana Press ; London : Springer [distributor], ©2013.
Series: Methods in molecular biology (Clifton, N.J.), 983.; Springer protocols (Series)
Edition/Format:   Print book : English : 2nd edView all editions and formats
Summary:

The book offers an introduction to the amoebozoa, presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence, and details molecular genetics  Read more...

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Genre/Form: Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Ludwig Eichinger; Francisco Rivero-Crespo
ISBN: 9781627033015 1627033017 9781627033022 1627033025
OCLC Number: 821702072
Description: xii, 479 pages : illustrations (some color) ; 26 cm.
Contents: Amoebozoa / Christina Schilde and Pauline Schaap --
Model organism Dictyostelium discoideum / Salvatore Bozzaro --
Comparative genomics of the dictyostelids / William F. Loomis --
One stop shop for everything Dictyostelium : dictyBase and the dicty stock center in 2012 / Petra Fey [and others] --
Fluorescent reporters and methods to analyze fluorescent signals / Annette Müller-Taubenberger and Hellen C. Ishikawa-Ankerhold --
Collection and cultivation of dictyostelids from the wild / Tracy E. Douglas [and others] --
Identification and verification of microRNAs by high-throughput sequencing / Jimmie Hällman [and others] --
Transcriptional profiling of Dictyostelium with RNA sequencing / Edward Roshan Miranda [and others] --
Analysis of chromatin organization by deep sequencing technologies / James L. Platt [and others] --
Pharmacogenetics of resistance to cisplatin and other anticancer drugs and the role of sphingolipid metabolism / Stephen Alexander, William S. Swatson, and Hannah Alexander --
N-glycomic and n-glycoproteomic studies in the social amoebae / Christa L. Feasley --
Measuring cheating, fitness, and segregation in Dictyostelium discoideum / Neil J. Buttery [and others] --
Application of the Cre-loxP system for generating multiple knock-out and knock-in targeted loci / Jan Faix [and others] --
Extrachromosomal inducible expression / Douwe M. Veltman and Peter J.M. Van Haastert --
Isolation of Dictyostelium nuclei for light and electron microscopy / Petros Batsios [and others] --
Investigation of DNA repair pathway activity / Anne-Marie C. Couto, Nicholas D. Lakin, and Catherine J. Pears --
Transcript localization in Dictyostelium discoideum cells by RNA FISH / Patrick Hofmann, Janis Kruse, and Christian Hammann --
Analysis of mitochondrial gene expression / Jessica E. Accari [and others] --
Mitochondrial respiratory complex function and the phenotypic consequences of dysfunction / Sarah J. Annesley [and others] --
Micropipette aspiration for studying cellular mechanosensory responses and mechanics / Yee-Seir Kee and Douglas N. Robinson --
Quantitative analysis of phagocytosis and phagosome maturation / Natascha Sattler, Roger Monroy, and Thierry Soldati --
Setting up and monitoring an infection of Dictyostelium discoideum with mycobacteria / Sonia Arafah [and others] --
Isolation of pathogen-containing vacuoles / Olga Shevchuk and Michael Steinert --
Immunomagnetic purification of fluorescent legionella-containing vacuoles / Ivo Finsel, Christine Hoffmann, and Hubert Hilbi --
Secretory lysosomes in Dictyostelium : visualization, characterization, and dynamics / Wanessa C. Lima and Pierre Cosson --
Monitoring autophagy in Dictyostelium / Ana Mesquita [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), 983.; Springer protocols (Series)
Responsibility: edited by Ludwig Eichinger, Francisco Rivero.

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