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Difference gel electrophoresis (DIGE) : methods and protocols

Author: Rainer Cramer; Reiner Westermeier
Publisher: New York : Humana Press : Springer, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 854.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This book introduces DIGE and its advantages in quantitative protein analysis, providing detailed protocols and important notes on the practical aspects of DIGE with both generic and specific  Read more...

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Genre/Form: Laboratory manuals
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Rainer Cramer; Reiner Westermeier
ISBN: 9781617795725 1617795720 9781617795732 1617795739
OCLC Number: 756594932
Description: xiii, 401 pages : illustrations (some color) ; 27 cm.
Contents: Part I Fundamentals --
DIGE : past and future / Jonathan S. Minden --
Basics of 2D DIGE / Phil Beckett --
Multifluorescence 2D gel imaging and image analysis / Ingo Vormbrock, Sonja Hartwig, and Stefan Lehr --
Assessing signal-to-noise in quantitative proteomics : multivariate statistical analysis in DIGE experiments / David B. Friedman --
Analysis of proteins using DIGE and MALDI mass spectrometry / Witold M. Winnik [and others] --
Synthesis and validation of cyanine-based dyes for DIGE / Michael E. Jung [and others] --
Part II Methods --
2D DIGE saturation labeling for minute sample amounts / Georg J. Arnold and Thomas Frohlich --
Proteomic analysis of redox-dependent changes using cysteine-labeling 2D DIGE / Hong-Lin Chan, John Sinclair, and John F. Timms --
Analysis of protein posttranslational modifications using DIGE-based proteomics / Robert M. DeKroon [and others] --
Comparative analyses of protein complexes by blue native DIGE / Katrin Peters and Hans-Peter Braun --
2D DIGE analysis of protein extracts from muscle tissue / Cecilia Gelfi and Sara De Palma --
Combination of highly efficient hexapeptide ligand library-based sample preparation with 2D DIGE for the analysis of the hidden human serum/plasma proteome / Sonja Hartwig and Stefan Lehr --
2D DIGE analysis of serum after fractionation by proteominertm beads / Cynthia Liang, Gek San Tan, and Maxey C.M. Chung --
Study design in DIGE-based biomarker discovery / Alexandra Graf and Rudolf Oehler --
Comparative 2D DIGE analysis of the depleted serum proteome for biomarker discovery / Megan Penno, Matthias Ernst, and Peter Hoffmann --
Part III Applications in clinical proteomics --
Differential gel-based proteomic approach for cancer biomarker discovery using human plasma / Keun Na [and others] --
2D DIGE for the analysis of RAMOS cells subproteomes / Marisol Fernandez and Juan Pablo Albar --
Application of saturation labeling in lung cancer proteomics / Gereon Poschmann [and others] --
Proteomic profiling of the epithelial-mesenchymal transition using 2D DIGE / Rommel A. Mathias, Hong Ji, and Richard J. Simpson --
Method for protein subfractionation of cardiovascular tissues before DIGE analysis / Athanasios Didangelos, Xiaoke Yin, and Manual Mayr --
Application of DIGE and mass spectrometry in the study of type 2 diabetes mellitus mouse models / Celia Smith, Davinia Mills, and Rainer Cramer --
Evaluating the efficacy of subcellular fractionation of blast cells using live cell labeling and 2D DIGE / Yin Ying Ho [and others] --
Part IV Applications in animal, plant, and microbial proteomics --
DIGE analysis of plant tissue proteomes using a phenolic protein extraction method / Christina Rode [and others] --
Native DIGE of fluorescent plant protein complexes / Veronika Reisinger and Lutz Andreas Eichacker --
Overview of 2D DIGE analysis of marine (environmental) bacteria / Ralf Rabus --
Application of 2D DIGE in animal proteomics / Ingrid Miller.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 854.; Springer protocols (Series)
Responsibility: edited by Rainer Cramer, Reiner Westermeier.

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