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DNA replication : methods and protocols
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DNA replication : methods and protocols

Auteur : Sonya Vengrova; Jacob Z Dalgaard
Éditeur : [New York?] : Humana ; [London : Springer, distributor], ©2009.
Collection : Methods in molecular biology (Clifton, N.J.), 521.; Springer protocols.
Édition/format :  Livre : AnglaisVoir toutes les éditions et les formats
Résumé :

Since the discovery of its structure, the field of DNA replication has expanded to cover a vast number of experimental systems. Here, expert researchers present techniques used to investigate DNA  Lire la suite...

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Détails

Genre/forme : Laboratory Manuals
Format : Livre
Tous les auteurs / collaborateurs : Sonya Vengrova; Jacob Z Dalgaard
ISBN : 9781603278140 1603278141 160327815X 9781603278157
Numéro OCLC : 262719643
Description : xiii, 692 p., [6] p. of plates : ill. (some col.) ; 27 cm.
Contenu : DNA replication initiation / Stephen J. Aves --
DNA replication fork proteins / Ulrich Hübscher --
Random and site-specific replication termination / Jacob Z. Dalgaard ... [et al.] --
Checkpoint regulation of DNA replication / Erik Boye, Henriette C. Skjølberg, and Beáta Grallert --
Introduction to molecular combing : genomics, DNA replication, and cancer / John Herrick and Aaron Bensimon --
Replication initiation point mapping : approach and implications / Sapna Das-Bradoo and Anja-Katrin Bielinsky --
Purification of restriction fragments containing replication intermediates from complex genomes for 2-D gel analysis / Larry D. Mesner, Pieter A. Dijkwel, and Joyce L. Hamlin --
Topological analysis of plasmid DNA replication intermediates using two-dimensional agarose gels / Olivier Hyrien --
Analysis of telomeric DNA replication using neutral-alkaline two-dimensional gel electrophoresis / Svetlana Makovets --
Chromatin immunoprecipitation of replication factors moving with the replication fork / Jordan B. Rapp ... [et al.] --
Density transfer as a method to analyze the progression of DNA replication forks / José Antonio Tercero --
High-resolution mapping of points of site-specific replication stalling / Sonya Vengrova and Jacob Z. Dalgaard --
DNA replication in nucleus-free xenopus egg extracts / Ronald Lebofsky, Tatsuro Takahashi, and Johannes C. Walter --
ChIP-chip to analyze the binding of replication proteins to chromatin using oligonucleotide DNA microarrays / Christopher J. Viggiani, Jennifer G. Aparicio, and Oscar M. Aparicio --
Analyzing origin activation patterns by copy number change experiments / Miruthubashini Raveendranathan and Anja-Katrin Bielinsky --
Detection of replication origins using comparative genomics and recombinational ARS assay / Conrad A. Nieduszynski and Anne D. Donaldson --
Isolation of restriction fragments containing origins of replication from complex genomes / Larry D. Mesner and Joyce L. Hamlin --
Application of alkaline sucrose gradient centrifugation in the analysis of DNA replication after DNA damage / Sascha Raschke, Jun Guan, and George Iliakis --
Isolation of recombinant DNA elongation proteins / Barbara van Loon, Elena Ferrari, and Ulrich Hübscher --
In vitro assays for studying helicase activities / Jeong-Hoon Kim and Yeon-Soo Seo --
The use of 2-aminopurine fluorescence to study DNA polymerase function / Linda J. Reha-Krantz --
Single-molecule observation of prokaryotic DNA replication / Nathan A. Tanner and Antoine M. van Oijen --
Visualization of DNA replication sites in mammalian nuclei / Daniela S. Dimitrova --
Cell-cycle synchrony for analysis of S. pombe DNA replication / Douglas D. Luche and Susan L. Forsburg --
Measuring DNA content by flow cytometry in fission yeast / Sarah A. Sabatinos and Susan L. Forsburg --
Microscopy techniques to examine DNA replication in fission yeast / Marc D. Green, Sarah A. Sabatinos, and Susan L. Forsburg --
Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe / Stephen E. Kearsey and Juraj Gregan --
Assays used to study the DNA replication checkpoint in fission yeast / Eishi Noguchi ... [et al.] --
Incorporation of thymidine analogs for studying replication kinetics in fission yeast / Nicholas Rhind --
The fast-halo assay for the assessment of DNA damage at the single-cell level / Piero Sestili --
Monitoring homologous recombination following replication fork perturbation in the fission yeast Schizosaccharomyces pombe / Fekret Osman and Matthew C. Whitby --
Computational methods to study kinetics of DNA replication / Scott Cheng-Hsing Yang, Michel G. Gauthier, and John Bechhoefer --
Use of DNA combing to study DNA replication in Xenopus and human cell-free systems / Kathrin Marheineke ... [et al.] --
Electron microscopy methods for studying in vivo DNA replication intermediates / Massimo Lopes --
Determining the replication dynamics of specific gene loci by single-molecule analysis of replicated DNA / Agnieszka Demczuk and Paolo Norio --
Use of DNA combing for studying DNA replication in vivo in yeast and mammalian cells / Etienne Schwob ... [et al.]
Titre de collection : Methods in molecular biology (Clifton, N.J.), 521.; Springer protocols.
Responsabilité : edited by Sonya Vengrova and Jacob Z. Dalgaard.

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