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DNA sequencing protocols

Author: Colin A Graham; Alison J M Hill
Publisher: Totowa, NJ : Humana Press, ©2001.
Series: Methods in molecular biology (Clifton, N.J.), v. 167.
Edition/Format:   eBook : Document : English : 2nd edView all editions and formats
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Manuels de laboratoire
Additional Physical Format: Print version:
DNA sequencing protocols.
Totowa, NJ : Humana Press, ©2001
(DLC) 00033483
(OCoLC)44045613
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Colin A Graham; Alison J M Hill
ISBN: 9781592591138 1592591132
OCLC Number: 170923856
Description: 1 online resource (x, 244 pages) : illustrations (some color).
Contents: Introduction to DNA sequencing / Colin A. Graham, Alison J.M. Hill --
The universal primers and the shotgun DNA sequencing method / Joachim Messing --
M13 sequencing / Qingzhong Yu --
Primer design and primer-directed sequencing / Ulrike Gerischer, Peter Durre --
Direct sequencing of DNA produced in a polymerase chain reaction / Ulrike Gerischer --
Solid phase flluorescent sequencing of the CFTR gene / Harry Cuppens, Jean-Jacques Cassiman --
Shotgun DNA sequencing / Alan T. Bankier --
Cycle sequencing / Robert W. Blakesley --
Cycle sequencing of polymerase chain reaction-amplified genomic DNA with dye-labeled universal primers / Johannes Zschocke, Georg F. Hoffman --
Automated fluorescent DNA sequencing on the ABI PRISM 377 / Jonathan R.E. MacBeath, Suzanna S. Harvey, Nicola J. Oldroyd --
Automated fluorescent DNA sequencing on the ABI PRISM 310 genetic analyzer / David Watts, Jonathan R.E. MacBeath --
Fluorescent sequencing protocols for the ALF / Shirley McQuaid --
Fluorescent sequencing for heterozygote mutation detection / Colin A. Graham, Alison J.M. Hill --
Sequence databases and the Internet / Anne E. Hughes --
DNA sequencing by capillary array electrophoresis / Norman J. Dovichi, JianZhong Zhang.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 167.
Responsibility: edited by Colin A. Graham and Allison J.M. Hill.

Abstract:

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many  Read more...

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"DNA Sequencing Protocols covers the commonly used protocols in a clearly written manner and is thorough in description. This book is highly recommended for all libraries serving researchers Read more...

 
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