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DNA topoisomerase protocols

Author: Mary-Ann Bjornsti; Neil Osheroff
Publisher: Totowa, N.J. : Humana Press, 1999-2001.
Series: Methods in molecular biology (Clifton, N.J.), v. 94-95.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Beginning with the Escherichia coli co protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes has been identified that catalyze changes in the linkage of DNA strands.

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Genre/Form: Laboratory Manuals
Laboratory manuals
Additional Physical Format: Online version:
DNA topoisomerase protocols.
Totowa, N.J. : Humana Press, 1999-2001
(OCoLC)642444159
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Mary-Ann Bjornsti; Neil Osheroff
ISBN: 0896035123 9780896035126 0896034445 9780896034440
OCLC Number: 40744357
Description: 2 volumes : illustrations ; 24 cm.
Contents: v. 1. DNA topology and enzymes --
v. 2. Enzymology and drugs. [v. 1.] Introduction to DNA topoisomerases / Mary-Ann Bjornsti, Neil Osheroff --
Resolution of DNA molecules by one-dimensional agarose-gel electrophoresis / Mary-Ann Bjornsti, Maureen D. Megonigal --
Two-dimensional agarose-gel electrophoresis of DNA topoisomers / Ryo Hanai, Joaquim Roca --
Analysis of altered DNA structures: cruciform DNA / Albert J. Courey --
Purification of supercoiled plasmid DNA / Kelly L. Jordan, Devon L. Evans, David J. Hall --
Purification and use of DNA minicircles with different linking numbers / Giorgio Camilloni, Rodolfo Negri, Micaela Caserta, Ernesto Mauro --
Isolation of kinetoplast DNA / Theresa A. Shapiro, Viiu A. Klein, Paul T. Englund --
Isolation of knotted DNA from coliphage P4 / Morten Isaksen, Bryan Julien, Richard Calendar, Björn H. Lindqvist --
Analysis of DNA knots and catenanes by agarose-gel electrophoresis / Stephen D. Levene, Hua Tsen --
Sedimentation analysis of bacterial nucleoid structure / Karl Drlica, Chang-Rung Chen, Samuel Kayman --
Coating DNA with RecA protein to distinguish DNA path by electron microscopy / E. Lynn Zechiedrich, Nancy J. Crisona --
Methods for analyzing DNA bending / Jason D. Kahn --
Formation of extrachromosomal DNA rings in Saccharomyces cerevisiae using site-specific recombination / Marc R. Gartenberg --
Overexpression and purification of bacterial DNA gyrase / Anthony Maxwell, Alison J. Howells --
Overexpression and purification of bacterial DNA topoisomerase I / Chang-Xi Zhu, Yuk-Ching Tse-Dinh --
Overexpression and purification of Escherichia coli DNA topoisomerase III / Russell J. DiGate --
Overexpression and purification of bacterial topoisomerase IV / Hong Peng, Kenneth J. Marians --
Purification of the bacteriophage T4 type II DNA topoisomerase / Kenneth N. Kreuzer, Sue H. Neece --
Overexpression and purification of DNA topoisomerase I from yeast / Mary-Ann Bjornsti, Jolanta Fertala --
Overexpression and purification of Saccharomyces cerevisiae DNA topoisomerase II from yeast / Janet E. Lindsley --
Purification of DNA topoisomerase II from Drosophila melanogaster / Stacie J. Froelich-Ammon, Paul S. Kingma, Neil Osheroff --
Purification of DNA topoisomerase I from human placenta / Joseph A. Holden --
Purification of baculovirus-expressed human DNA topoisomerase I / Lance Stewart, James J. Champoux --
Rapid purification of DNA topoisomerase II containing a hexahistidine tag by metal ion affinity chromatography / Harald Biersack, Sanne Jensen, Ole Westergaard --
Metabolic labeling, immunoprecipitation, and two-dimensional tryptic phosphopeptide mapping of human topoisomerase II / Nicholas J. Wells, Ian D. Hickson --
Immunoblot analysis and band depletion assays / Scott H. Kaufmann, Phyllis A. Svingen --
Visualization of DNA topoisomerases by electron microscopy / Piero Benedetti, Alessandra Silvestri --
Compendium of DNA topoisomerase sequences / Paul R. Caron. [v. 2.] Assaying DNA topoisomerase I relaxation activity / Lance Stewart, James J. Champoux --
DNA topoisomerase II-catalyzed DNA decatenation / Andrea Haldane, Daniel M. Sullivan --
Plasmid DNA supercoiling by DNA gyrase / Penny J. Sayer, Martin L. Goble, Mark Oram, L. Mark Fisher --
Analyzing reverse gyrase activity / Michel Duguet, Christine Jaxel, Anne-Cécile Déclais, Fabrice Confalonieri, Janine Marsault, Claire Bouthier Tour, Marc Nadal, Christiane Portemer --
Topoisomerase II-catalyzed ATP hydrolysis as monitored by thin-layer chromatography / Paul S. Kingma, John M. Fortune, Neil Osheroff --
Use of a real-time, coupled assay to measure the ATPase activity of DNA topoisomerase II / Janet E. Lindsley --
Analysis of topoisomerase-DNA interactions by electrophoretic mobility shift assay / Stewart Shuman --
Filter binding assays for topoisomerase-DNA complexes / Joaquim Roca --
DNA topoisomerase I-mediated nicking of circular duplex DNA / James J. Champoux --
Analysis of cleavage complexes using reactive inhibitor derivatives / Kenneth N. Kreuzer, Catherine H. Freudenreich, Yves Pommier --
Uncoupling of topoisomerase-mediated DNA cleavage and religation / Anni H. Andersen, Kent Christiansen, Ole Westergaard --
Synthesis and use of DNA containing a 5'-bridging phosphorothioate as a suicide substrate for Type I DNA topoisomerases / Alex B. Burgin --
Isolation of covalent enzyme-DNA complexes / Thomas C. Rowe, Dale Grabowski, Ram Ganapathi --
ICE bioassay : isolating in vivo complexes of enzyme to DNA / Deepa Subramanian, Christine Sommer Furbee, Mark T. Muller --
DNA-unwinding test using eukaryotic DNA topoisomerase I / Linus L. Shen --
Drug-DNA interactions / David E. Graves --
Quinolone interactions with DNA and DNA gyrase / Linus L. Shen --
Bactericidal assays for fluoroquinolones / Thomas D. Gootz --
Drug toxicity in E. coli cells expressing human topoisomerase I / Scott T. Taylor, Rolf Menzel --
Drug-induced cytotoxicity in tissue culture / Cynthia E. Herzog, Leonard A. Zwelling --
Analysis of drug uptake-flow cytometry / Grace Zoorob, Thomas Burke --
Cell-cycle analysis of drug-treated cells / Frank Traganos, Gloria Juan, Zbigniew Darzynkiewicz --
Assaying drug-induced apoptosis / Zbigniew Darzynkiewicz, Gloria Juan, Frank Traganos --
Single-strand conformational polymorphism analysis of DNA topoisomerases I and II / Mary K. Danks --
PCR-based cloning of DNA topoisomerase genes / Wai Mun Huang --
Topoisomerase II-catalyzed relaxation and catenation of plasmid DNA / John M. Fortune, Neil Osheroff --
Topoisomerase II-mediated cleavage of plasmid DNA / D. Andrew Burden, Stacie J. Froelich-Ammon, Neil Osheroff --
Drug-induced stabilization of covalent DNA topoisomerase I-DNA intermediates : DNA cleavage assays / Paola Fiorani, Christine L. Hann, Piero Benedetti, Mary-Ann Bjornsti --
Studying DNA topoisomerase I-targeted drugs in the yeast : Saccharomyces cerevisiae / Michael H. Woo, John R. Vance, Mary-Ann Bjornsti --
Yeast systems for demonstrating the targets of anti-topoisomerase II agents / John L. Nitiss, Karin C. Nitiss.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 94-95.
Responsibility: edited by Neil Osheroff and Mary-Ann Bjornsti.
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"...an invaluable practical aid. The central and expanding role of these enzymes in basic cell biology together with their important pharmaceutical applications will ensure that book will remain Read more...

 
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