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DNA viruses : methods and protocols

Author: Paul M Lieberman
Publisher: Totowa, N.J. : Humana Press, ©2005.
Series: Methods in molecular biology (Clifton, N.J.), v. 292.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches.
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Genre/Form: Laboratory manuals
Manuels de laboratoire
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Paul M Lieberman
ISBN: 158829353X 9781588293534 159259848X 9781592598489
OCLC Number: 55503457
Description: xiv, 498 pages : illustrations (some color) ; 23 cm.
Contents: Viral detection --
Quantitative detection of Epstein-Barr virus DNA in clinical specimens by rapid real-time PCR targeting a highly conserved region of EBNA-1 --
Profiling of Epstein-Barr virus latent RNA expression in clinical specimens by gene-specific multiprimed cDNA synthesis and PCR --
Quantitative detection of viral gene expression in populations of Epstein-Barr virus-infected cells in vivo --
Detection and quantification of the rare latently infected cell undergoing herpes simplex virus transcriptional activation in the nervous system in vivo --
Reporter cell lines for the detection of herpes simplex viruses --
Unraveling the architecture of viruses by high-resolution atomic force microscopy --
Studying the structure of large viruses with multiresolution imaging --
Herpes simplex virus-cell interactions studied by low-fading contrasted immunofluorescence --
Herpes simplex virus-cell interactions studied by immunogold cryosection electron microscopy --
FTIR microscopy detection of cells infected with viruses --
The JC virus-like particle overlay assay --
Analysis of fusion using a virus-free cell fusion assay --
Pseudovirions as specific tools for investigation of virus interactions with cells --
Simultaneous in situ detection of RNA, DNA, and protein using tyramide-coupled immunofluorescence --
Identification and characterization of herpesviral immediate-early genes --
Methods for measuring the replication and segregation of Epstein-Barr virus-based plasmids --
DNA affinity purification of Epstein-Barr virus OriP-binding proteins. Pre-B-cell colony formation assay --
Luciferase real-time bioluminescence imaging for the study of viral pathogenesis --
Culturing primary and transformed neuronal cells for studying pseudorabies virus infection --
Human papillomavirus type 31 life cycle: methods for study using tissue culture models --
Molecular genetics of herpesviruses: a recombinant technology approach --
Molecular genetics of DNA viruses: recombinant virus technology --
Genetic analysis of cytomegalovirus by shuttle mutagenesis --
Construction of a gene inactivation library for Bovine herpesvirus 1 using infectious clone technology --
Selective silencing of viral gene E6 and E7 expression in HPV-positive human cervical carcinoma cells using small interfering RNAs --
Design of a herpes simplex virus type 2 long oligonucleotide-based microarray: global analysis of HSV-2 transcript abundance during productive infection --
Real-time quantitative PCR analysis of viral transcription --
Rapid screening of chemical inhibitors that block processive DNA synthesis of herpesviruses: potential application to high-throughput screening.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 292.
Responsibility: edited by Paul M. Lieberman.
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Abstract:

Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and  Read more...

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"Good presentation...laboratories working with DNA viruses will find ample useful material...." - Doody's Health Sciences Book Review Journal

 
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