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Eicosanoid protocols

Author: Elias A Lianos
Publisher: Totowa, N.J. : Humana Press, ©1999.
Series: Methods in molecular biology (Clifton, N.J.), v. 120.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Eicosanoid protocols.
Totowa, N.J. : Humana Press, ©1999
(DLC) 98052322
(OCoLC)40621729
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Elias A Lianos
ISBN: 0585237689 9780585237688 1592592635 9781592592630 9780896036673 0896036677
OCLC Number: 45729205
Description: 1 online resource (xi, 296 pages) : illustrations.
Contents: Use of a Retroviral Vector for Expression of a Foreign Gene / Brent W. Miller and Aubrey R. Morrison --
Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues / William S. Powell --
Assessment of Cyclooxygenase RNA Expression by Northern Hybridization / Zhonghong Guan and Aubrey R. Morrison --
Immunohistochemical Analysis of Cyclooxygenase Expression in Human Skin / ShaAvhree Y. Buckman [and others] --
Separation of Hydroxyeicosatetraenoic Acids and Leukotrienes Originating from Kidney Tissue by C-18 Reverse-Phase High-Pressure Liquid Chromatography / Mukut Sharma, Beatrice Hucke, and Elias A. Lianos --
Localization and Distribution of Cyclooxygenase-2 in Brain Tissue by Immunohistochemistry / M. Kerry O'Banion and John A. Olschowka --
Assessment of Cyclooxygenase Protein Expression by Western Blotting / Zhonghong Guan and Aubrey R. Morrison --
Assessment of Arachidonic Acid Distribution into Phospholipids of Inflammatory Cells / Alfred N. Fonteh --
Determination of Phospholipase A2s Expression in Mast Cells by Reverse-Transcriptase Polymerase Chain Reaction / Alfred N. Fonteh, William Reed, and James M. Samet --
Enzyme Immunoassays (EIA) of Eicosanoids / Kingsley Yin and Lloyd J. Forman --
Transcellular Regulation of Eicosanoid Biosynthesis / Karsten Gronert [and others] --
Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry / Samuel C. Blackman [and others] --
Epoxyeicosatrienoic Acids : Biosynthesis, Regulation, and Actions / John D. Imig --
Assessment of the Expression of Prostaglandin Synthase-2 in Swiss 3T3 Fibroblasts / Srinivasa T. Reddy and Harvey R. Herschman --
Assessment of 5-Lipoxygenase Activity and Cellular Distribution / Robert A. Lepley --
Leukotrienes, Lipoxins, and Hydroxyeicosatetraenoic Acids / Eric G. Spokas, Joshua Rokach, and Patrick Y.-K. Wong --
Whole Blood Assays for Evaluation of Thromboxane Synthase Inhibition / Eric G. Spokas [and others] --
Novel Eicosanoids: Isoprostanes and Related Compounds / L. Jackson Roberts II [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), v. 120.
Responsibility: edited by Elias A. Lianos.

Abstract:

This readily reproducible collection of methods for the study of eicosanoids concentrates on studying the regulation of expression and function of enzymes, particularly cyclooxygenase, phospholipase  Read more...

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"...well-produced, timely book..."-The Quarterly Review of Biology"A very useful book for all specialists on eicosanoid acids, which was lacking until now in the literature." - Cellular and Molecular Read more...

 
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