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Environmental monitoring of bacteria

Author: Clive Edwards
Publisher: Totowa, N.J. : Humana Press, ©1999.
Series: Methods in biotechnology, 12.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This text brings together a collection of detailed molecular methods for the environmental monitoring of bacterial populations. These methods include techniques for the recovery of cells, for the  Read more...

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Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Clive Edwards
ISBN: 0896035662 9780896035669
OCLC Number: 40759338
Description: x, 333 pages : illustrations ; 24 cm.
Contents: Some problems posed by natural environments for monitoring microorganisms --
Sampling sediment and soil: use of coring devices --
Sampling water bodies: tangential flow filtration --
Dielectrophoresis --
Flow cytometry and cell sorting: rapid analysis and separation of individual bacterial cells from natural environments --
Magnetic particle-based separation techniques for monitoring bacteria from natural environments --
DNA extraction from natural enviornments --
Automated sequencing of DNA retrieved from environmental samples --
Analysis of DNA sequences --
Fluorescent polymerase chanin reaction/restriction fragment lenght polymorphism monitoring of genes amplified directly from bacterial communities in soils and sediments --
Recovery and analysis of ribosomal RNA sequences from the environment --
Application of denaturing gradient gel electrophoresis to microbial ecology --
Reporter gene expression for monitoring microorganisms --
Characterizing microorganisms in the environment by fatty acid analysis --
Fluorescent in situ hybridization and the analysis of the single cell --
Specific detection, viability assessment, and macromolecular staining of bacteria for flow cytometry --
Confocal laser scanning microscopy of environmental samples --
Monitoring microbial activities using membrane inlet mass spectrometry --
Experimental biofilms and their applications in the study of environmental processes --
Establishment of experimental biofilms using the modified robbins device and flow cells.
Series Title: Methods in biotechnology, 12.
Responsibility: edited by Clive Edwards.
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"This book looks at state-of-the-art techniques for monitoring bacteria in natural environments, offers step-by-step instructions to ensure readily reproducible results, and provides numerous tips on Read more...

 
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