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Epidermal cells : methods and protocols

Author: Kursad Turksen
Publisher: New York, NY : Humana Press, ©2010.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), volume 585.
Edition/Format:   eBook : Document : English : 2nd edView all editions and formats
Database:WorldCat
Summary:

This highly practical book describes in step-by-step detail the powerful methods developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These  Read more...

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Additional Physical Format: Print version:
Epidermal cells.
New York, NY : Humana Press, ©2010
(DLC) 2009935434
(OCoLC)401157592
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Kursad Turksen
ISBN: 9781607613800 1607613808
OCLC Number: 468823938
Description: 1 online resource (xv, 446 pages) : illustrations (some color).
Contents: Isolation of adult mouse stem keratinocytes using magnetic cell sorting (MACS) / Corina Lorz [and others] --
Functional investigations of keratinocyte stem cells and progenitors at a single-cell level using multiparallel clonal microcultures / Nicolas O. Furtunel [and others] --
Growth and stratification of epithelial cells in minimal culture conditions / Federica Riva [and others] --
Matched cultures of keratinocytes and fibroblasts derived from normal and NER-deficient mouse models / Alex Pines and Claude Backendorf --
Establishment of spontaneously immortalized keratinocyte lines from wild-type and mutant mice / Julia Reichelt and Ingo Haase --
Study of epidermal differentiation in human kertinocytes cultured in autocrine conditions / Frédéric Minner, Franc̦oise Herphelin, and Yves Poumay --
Directed differentiation of human embryonic stem cells to epidermal progenitors / Christian M. Metallo [and others] --
Expression and analysis of exogenous proteins in epidermal cells / Lina Dagnino, Ernest Ho, and Wing Y. Change --
Using siRNA knockdown in HaCaT cells to study transcriptional control of epidermal proliferation potential / Julie Wells and Xing Dai --
RNA interference in keratinocytes and an organotypic model of human epidermis / Cory L. Simpson, Shin-ichiro Kojima, and Spiro Getsios --
Scanning for transcription factor binding by a variant EMSA / Igor Gurevich, Carmen Zhang, and Brian J. Aneskievich --
Chromatin immunoprecipitation for identifying transcription factor targets in kertinocytes / Kori Ortt and Satrajit Sinha --
Gene expression profiling of mouse epidermal keratinocytes / Ramón García-Escudero and Jesús M. Paramio --
Analysis of tissue-specific gene expression using laser capture microdissection / Martin Ruetze [and others] --Comprehensive transcriptional profiling of human epidermis, reconstituted epidermal equivalents, and cultured keratinocytes using DNA microarray chips / Din-Dar Lee [and others] --
Molecular profiling of the epidermis : a proteomics approach / Jianjun Shen and Susan M. Fischer --
Detection of gene expression in embryonic tissues and stratified epidermis by in situ hybridization / Maria I. Morasso --
Embryonic mammary anlagen analysis using immunolabelling of whole mounts / Heena Panchal, Olivia Wansbury, and Beatrice A. Howard --
Whole-mount assays for gene induction and barrier formation in the developing epidermis / Carolyn Byrne [and others] --
Tetracycline-regulated gene expession in transgenic mouse epidermis / Rose-Anne Romano and Satrajit Sinha --
A versatile murine 3D organotypic model to evaluate aspects of wound healing and epidermal organization / Eve Kandyba, Malcolm Hodgins, and Patricia Martin --
Optical and biochemical dissection of connexin and disease-linked connexin mutants in 3D organotypic epidermis / Stéphanie Langlois, Jared M. Churko, and Dale W. Laird --
Cytokine release in tissue-engineered epidermal equivalents after prolonged mechanical loading / Lisette H. Cornelissen [and others] --
Three-dimensional human tissue models of wounded skin / Christophe Egles, Jonathan A. Garlick, and Yulia Shamis --
In vivo transplantation of genetically modified mouse embryonic epidermis / Ana Belén Martínez-Cruz [and others] --
A transplant model for human epidermal skin regeneration / Sophie Paquet-Fifield [and others] --
Identification of epithelial stem cells in vivo and in vitro using keratin 19 and brdU / Danielle Larouche [and others] --
Isolation and culture of hair follicle pluripotent stem (hfPS) cells and their use for nerve and spinal cord regeneration / Yasuyuki Amoh and Robert M. Hoffman --
Limiting dilution analysis of murine epidermal stem cells using an in vivo regeneration assay / Lauren R. Strachan and Ruby Ghadially.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), volume 585.
Responsibility: edited by Kursad Turksen.
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From the reviews of the second edition: "This book introduces a wide variety of laboratory protocols for isolating and analyzing the differentiation potential of mammalian epidermal cells. ... The Read more...

 
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