skip to content
Exon skipping : methods and protocols Preview this item
ClosePreview this item
Checking...

Exon skipping : methods and protocols

Author: Annemieke Aartsma-Rus
Publisher: New York : Humana Press, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 867.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
"Next generation" sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in  Read more...
Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

 

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Printed edition:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Annemieke Aartsma-Rus
ISBN: 9781617797675 1617797677 1617797669 9781617797668
OCLC Number: 785149446
Language Note: English.
Notes: Includes index.
Description: 1 online resource (xi, 440 pages).
Contents: DNA diagnostics and exon skipping / Umasuthan Srirangalingam and Shern L. Chew --
Bioinformatics and mutations leading to exon skipping / F.O. Desmet and C. Béroud --
Minigenes to confirm exon skipping mutations / Lourdes R. Desviat, Belén Pérez, and Magdalena Ugarte --
Analysis and interpretation of RNA splicing alterations in genes involved in genetic disorders / Maaike P.G. Vreeswijk and Heleen M. van der Klift --
Exon skipping mutations in neurofibromatosis / Emanuele Buratti and Diana Baralle --
Overview on applications of antisense-mediated exon skipping / Willeke M.C. van Roon-Mom and Annemieke Aartsma-Rus --
Overview on DMD exon skipping / Annemieke Aartsma-Rus --
Overview on AON design / Annemieke Aartsma-Rus --
Optimizing RNA/ENA chimeric antisense oligonucleotides using in vitro splicing / Yasuhiro Takeshima, Mariko Yagi, and Masafumi Matsuo --
Optimizing antisense oligonucleotides using phophorodiamidate morpholino oligomers / Linda J. Popplewell, Alberto Malerba, and George Dickson --
Optimizing splice-switching oligomer sequences using 2'-O-methyl phosphorothioate chemistry / Carl Adkin, Sue Fletcher, and Steve D. Wilton --
Exon skipping quantification by real-time PCR / Alessandra Ferlini and Paola Rimessi --
Antisense-mediated exon skipping to shift alternative splicing to treat cancer / Jing Wan --
Antisense-mediated exon skipping to generate soluble receptors / A. Seda Yilmaz-Elis and J. Sjef Verbeek --
Antisense-mediated exon skipping to reframe transcripts / Sandrina Turczynski [and others] --
U1 snRNA as an effective vector for stable expression of antisense molecules and for the inhibition of the splicing reaction / Julie Martone, Fernanda Gabriella De Angelis, and Irene Bozzoni --
Engineering U7snRNA gene to reframe transcripts / Aurélie Goyenvalle --
Dynamic fluorescent and luminescent reporters for cell-based splicing screens / Claude C. Warzecha, Ruben Hovhannisyan, and Russ P. Carstens --
Antisense-mediated exon-skipping to induce gene knockdown / Petra Disterer and Bernard Khoo --
Antisense-mediated exon inclusion / Yimin Hua and Adrian R. Krainer --
Antisense genes to induce exon inclusion / Rachel Nlend Nlend and Daniel Schümperli --
Using mini-genes to identify factors that modulate alternative splicing / Robert Morse, Adrian G. Todd, and Philip J. Young --
Overview of alternative oligonucleotide chemistries for exon skipping / Amer F. Saleh, Andrey A. Arzumanov, and Michael J. Gait --
Identification of peptides for tissue-specific delivery / Hans Heemskerk --
Systemic delivery of antisense oligomer in animal models and its implications for treating DMD / Qi Long Lu and Bo Wu --
Cell-penetrating peptides enhance systemic delivery of antisense morpholino oligomers / Hong M. Moulton --
Optimizing tissue-specific antisense oligonucleotide-peptide conjugates / Corinne A. Betts [and others].
Series Title: Methods in molecular biology (Clifton, N.J.), v. 867.
Responsibility: edited by Annemieke Aartsma-Rus.

Abstract:

This Methods in Molecular Biology (TM) book reviews methods and techniques used for exon skipping, such as methods on how to distinguish "real polymorphisms" from mutations that affect splicing.  Read more...

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/785149446> # Exon skipping : methods and protocols
    a schema:MediaObject, schema:CreativeWork, schema:Book ;
    library:oclcnum "785149446" ;
    library:placeOfPublication <http://id.loc.gov/vocabulary/countries/nyu> ;
    library:placeOfPublication <http://dbpedia.org/resource/New_York_City> ; # New York
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genetics> ; # Genetics
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/natural_science_disciplines> ; # Natural Science Disciplines
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genetic_structures> ; # Genetic Structures
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/investigative_techniques> ; # Investigative Techniques
    schema:about <http://dewey.info/class/572.86/e23/> ;
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Thing/medicine> ; # Medicine.
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/publication_formats> ; # Publication Formats
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Thing/biomedicine> ; # Biomedicine.
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/exons> ; # Exons
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Thing/human_genetics> ; # Human genetics.
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/publication_characteristics> ; # Publication Characteristics
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genes> ; # Genes
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/phenomena_and_processes> ; # Phenomena and Processes
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/analytical_diagnostic_and_therapeutic_techniques_and_equipment> ; # Analytical, Diagnostic and Therapeutic Techniques and Equipment
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/laboratory_manuals> ; # Laboratory Manuals
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/exons_genetics> ; # Exons (Genetics)
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/gene_components> ; # Gene Components
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/disciplines_and_occupations> ; # Disciplines and Occupations
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genetic_phenomena> ; # Genetic Phenomena
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/biological_science_disciplines> ; # Biological Science Disciplines
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genome> ; # Genome
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genome_components> ; # Genome Components
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genetic_techniques> ; # Genetic Techniques
    schema:about <http://experiment.worldcat.org/entity/work/data/1100177639#Topic/biology> ; # Biology
    schema:bookFormat schema:EBook ;
    schema:contributor <http://viaf.org/viaf/251877187> ; # Annemieke Aartsma-Rus
    schema:copyrightYear "2012" ;
    schema:datePublished "2012" ;
    schema:description "DNA diagnostics and exon skipping / Umasuthan Srirangalingam and Shern L. Chew -- Bioinformatics and mutations leading to exon skipping / F.O. Desmet and C. Béroud -- Minigenes to confirm exon skipping mutations / Lourdes R. Desviat, Belén Pérez, and Magdalena Ugarte -- Analysis and interpretation of RNA splicing alterations in genes involved in genetic disorders / Maaike P.G. Vreeswijk and Heleen M. van der Klift -- Exon skipping mutations in neurofibromatosis / Emanuele Buratti and Diana Baralle -- Overview on applications of antisense-mediated exon skipping / Willeke M.C. van Roon-Mom and Annemieke Aartsma-Rus -- Overview on DMD exon skipping / Annemieke Aartsma-Rus -- Overview on AON design / Annemieke Aartsma-Rus -- Optimizing RNA/ENA chimeric antisense oligonucleotides using in vitro splicing / Yasuhiro Takeshima, Mariko Yagi, and Masafumi Matsuo -- Optimizing antisense oligonucleotides using phophorodiamidate morpholino oligomers / Linda J. Popplewell, Alberto Malerba, and George Dickson -- Optimizing splice-switching oligomer sequences using 2'-O-methyl phosphorothioate chemistry / Carl Adkin, Sue Fletcher, and Steve D. Wilton -- Exon skipping quantification by real-time PCR / Alessandra Ferlini and Paola Rimessi -- Antisense-mediated exon skipping to shift alternative splicing to treat cancer / Jing Wan -- Antisense-mediated exon skipping to generate soluble receptors / A. Seda Yilmaz-Elis and J. Sjef Verbeek -- Antisense-mediated exon skipping to reframe transcripts / Sandrina Turczynski [and others] -- U1 snRNA as an effective vector for stable expression of antisense molecules and for the inhibition of the splicing reaction / Julie Martone, Fernanda Gabriella De Angelis, and Irene Bozzoni -- Engineering U7snRNA gene to reframe transcripts / Aurélie Goyenvalle -- Dynamic fluorescent and luminescent reporters for cell-based splicing screens / Claude C. Warzecha, Ruben Hovhannisyan, and Russ P. Carstens -- Antisense-mediated exon-skipping to induce gene knockdown / Petra Disterer and Bernard Khoo -- Antisense-mediated exon inclusion / Yimin Hua and Adrian R. Krainer -- Antisense genes to induce exon inclusion / Rachel Nlend Nlend and Daniel Schümperli -- Using mini-genes to identify factors that modulate alternative splicing / Robert Morse, Adrian G. Todd, and Philip J. Young -- Overview of alternative oligonucleotide chemistries for exon skipping / Amer F. Saleh, Andrey A. Arzumanov, and Michael J. Gait -- Identification of peptides for tissue-specific delivery / Hans Heemskerk -- Systemic delivery of antisense oligomer in animal models and its implications for treating DMD / Qi Long Lu and Bo Wu -- Cell-penetrating peptides enhance systemic delivery of antisense morpholino oligomers / Hong M. Moulton -- Optimizing tissue-specific antisense oligonucleotide-peptide conjugates / Corinne A. Betts [and others]."@en ;
    schema:description ""Next generation" sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate "real polymorphisms" from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping."@en ;
    schema:exampleOfWork <http://worldcat.org/entity/work/id/1100177639> ;
    schema:genre "Laboratory Manuals"@en ;
    schema:genre "Laboratory manuals"@en ;
    schema:genre "Electronic books"@en ;
    schema:inLanguage "en" ;
    schema:isPartOf <http://worldcat.org/issn/1064-3745> ; # Methods in molecular biology (Clifton, N.J.) ;
    schema:isSimilarTo <http://worldcat.org/entity/work/data/1100177639#CreativeWork/> ;
    schema:name "Exon skipping : methods and protocols"@en ;
    schema:productID "785149446" ;
    schema:publication <http://www.worldcat.org/title/-/oclc/785149446#PublicationEvent/new_york_humana_press_2012> ;
    schema:publisher <http://experiment.worldcat.org/entity/work/data/1100177639#Agent/humana_press> ; # Humana Press
    schema:url <https://link.springer.com/openurl?genre=book&isbn=978-1-61779-766-8> ;
    schema:url <http://dx.doi.org/10.1007/978-1-61779-767-5> ;
    schema:url <http://link.springer.com/openurl?genre=book&isbn=978-1-61779-767-5> ;
    schema:url <http://link.springer.com/openurl?genre=book&isbn=978-1-61779-766-8> ;
    schema:url <http://www.springerprotocols.com/doiresolver?genre=book&pid=10.1007/978-1-61779-767-5> ;
    schema:url <http://link.springer.com/10.1007/978-1-61779-767-5> ;
    schema:workExample <http://worldcat.org/isbn/9781617797675> ;
    schema:workExample <http://worldcat.org/isbn/9781617797668> ;
    schema:workExample <http://dx.doi.org/10.1007/978-1-61779-767-5> ;
    wdrs:describedby <http://www.worldcat.org/title/-/oclc/785149446> ;
    .


Related Entities

<http://dbpedia.org/resource/New_York_City> # New York
    a schema:Place ;
    schema:name "New York" ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Thing/human_genetics> # Human genetics.
    a schema:Thing ;
    schema:name "Human genetics." ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/analytical_diagnostic_and_therapeutic_techniques_and_equipment> # Analytical, Diagnostic and Therapeutic Techniques and Equipment
    a schema:Intangible ;
    schema:name "Analytical, Diagnostic and Therapeutic Techniques and Equipment"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/biological_science_disciplines> # Biological Science Disciplines
    a schema:Intangible ;
    schema:name "Biological Science Disciplines"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/disciplines_and_occupations> # Disciplines and Occupations
    a schema:Intangible ;
    schema:name "Disciplines and Occupations"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/exons_genetics> # Exons (Genetics)
    a schema:Intangible ;
    schema:name "Exons (Genetics)"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genetic_phenomena> # Genetic Phenomena
    a schema:Intangible ;
    schema:name "Genetic Phenomena"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genetic_structures> # Genetic Structures
    a schema:Intangible ;
    schema:name "Genetic Structures"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genetic_techniques> # Genetic Techniques
    a schema:Intangible ;
    schema:name "Genetic Techniques"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/genome_components> # Genome Components
    a schema:Intangible ;
    schema:name "Genome Components"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/investigative_techniques> # Investigative Techniques
    a schema:Intangible ;
    schema:name "Investigative Techniques"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/laboratory_manuals> # Laboratory Manuals
    a schema:Intangible ;
    schema:name "Laboratory Manuals"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/natural_science_disciplines> # Natural Science Disciplines
    a schema:Intangible ;
    schema:name "Natural Science Disciplines"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/phenomena_and_processes> # Phenomena and Processes
    a schema:Intangible ;
    schema:name "Phenomena and Processes"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/publication_characteristics> # Publication Characteristics
    a schema:Intangible ;
    schema:name "Publication Characteristics"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1100177639#Topic/publication_formats> # Publication Formats
    a schema:Intangible ;
    schema:name "Publication Formats"@en ;
    .

<http://link.springer.com/10.1007/978-1-61779-767-5>
    rdfs:comment "Springer Complete Protocols" ;
    .

<http://viaf.org/viaf/251877187> # Annemieke Aartsma-Rus
    a schema:Person ;
    schema:familyName "Aartsma-Rus" ;
    schema:givenName "Annemieke" ;
    schema:name "Annemieke Aartsma-Rus" ;
    .

<http://worldcat.org/entity/work/data/1100177639#CreativeWork/>
    a schema:CreativeWork ;
    schema:description "Printed edition:" ;
    schema:isSimilarTo <http://www.worldcat.org/oclc/785149446> ; # Exon skipping : methods and protocols
    .

<http://worldcat.org/isbn/9781617797668>
    a schema:ProductModel ;
    schema:isbn "1617797669" ;
    schema:isbn "9781617797668" ;
    .

<http://worldcat.org/isbn/9781617797675>
    a schema:ProductModel ;
    schema:isbn "1617797677" ;
    schema:isbn "9781617797675" ;
    .

<http://worldcat.org/issn/1064-3745> # Methods in molecular biology (Clifton, N.J.) ;
    a bgn:PublicationSeries ;
    schema:hasPart <http://www.worldcat.org/oclc/785149446> ; # Exon skipping : methods and protocols
    schema:issn "1064-3745" ;
    schema:name "Methods in molecular biology (Clifton, N.J.) ;" ;
    schema:name "Methods in molecular biology," ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.