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Forensic DNA biology : a laboratory manual

Author: Kelly M Elkins
Publisher: Waltham, MA : Academic Press, 2013.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:
A collection of forensic DNA typing laboratory experiments designed for academic and training courses at the collegiate level.
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Genre/Form: Laboratory Manuals
Document Type: Book
All Authors / Contributors: Kelly M Elkins
ISBN: 9780123945853 0123945852
OCLC Number: 811349789
Description: xxvi, 198 pages : illustrations ; 24 cm
Contents: 1. Pipetting 2. Serology 3. Sampling Biological Evidence for DNA Extraction 4. DNA Extraction 5. Determination of quality and quantity of DNA using agarose gel electrophoresis 6. Determination of DNA quality and quantity using UV-vis spectroscopy 7. Determination of DNA quantity by fluorescence spectroscopy 8. Real-time polymerase chain reaction (PCR) quantitation of DNA 9. Multiplex polymerase chain reaction (PCR) primer design (in silico) 10. Testing designed PCR primers in multiplex reactions 11. Multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci using a commercial kit 12. Capillary electrophoresis of short-tandem repeat (STR) polymerase chain reaction (PCR) products from a commercial multiplex kit 13. Computing random match probability from DNA profile data using population databases 14. Mitochondrial deoxyribonucleic acid (mtDNA) single nucleotide polymorphism (SNP) detection 15. Analysis of deoxyribonucleic acid (DNA) sequence data using bioedit 16. Ribonucleic acid (RNA) extraction 17. Y-STR PCR amplification 18. Human genetic analysis: paternity or missing persons cases and statistics 19. Low copy number stochastic results 20. Using in silico methods to construct a short tandem repeat (STR) deoxyribonucleic acid (DNA) sequence for cloning 21. Deoxyribonucleic acid (DNA) extraction from botanical material and polymerase chain reaction (PCR) amplification 22. Social, ethical and regulatory concerns 23. Selected forensic DNA biology case studies Appendix: Allele frequencies of CODIS loci
Responsibility: Kelly M. Elkins.

Abstract:

DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. This title provides practical instruction on  Read more...

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"This manual presents a collection of novel laboratory experiments intended for advanced undergraduate students and those new to the field of forensic DNA biology and DNA typing." --Evidence Read more...

 
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