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Functional analysis of bacterial genes : a practical manual

Author: Wolfgang Schumann, Prof. Dr. rer. nat.; S Dusko Ehrlich; Naotake Ogasawara
Publisher: Chichester ; New York : John Wiley & Sons, 2001.
Edition/Format:   Print book : EnglishView all editions and formats
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Genre/Form: Laboratory manuals
Additional Physical Format: Online version:
Functional analysis of bacterial genes.
Chichester ; New York : John Wiley & Sons, 2001
(OCoLC)761228090
Document Type: Book
All Authors / Contributors: Wolfgang Schumann, Prof. Dr. rer. nat.; S Dusko Ehrlich; Naotake Ogasawara
ISBN: 0471490083 9780471490081
OCLC Number: 123102660
Description: xxiii, 373 pages : illustrations ; 24 cm
Contents: Aims of the functional analysis project --
In silico genome analysis --
Inactivation of Bacillus subtilis genes without known functions --
Analysis of essential genes --
Transcriptional analysis of large regions of the bacterial genome --
Useful methods for transcriptional analysis --
Northern blot analysis with non-RI detection systems --
Micado, an integrative database dedicated to the functional analysis of Bacillus subtilis and microbial genomics --
Phenotype responses and reporter gene activity from the systematic functional analysis of Bacillus subtilis unknown genes --
The contribution of the EC consortium to the two-dimensional protein index of Bacillus subtilis --
Whole-cell fingerprinting --
pyrolysis mass spectrometry --
Metabolism of small molecules and inorganics --
Screening for genes involved in nucleotide and nitrogen metabolism --
Screening for growth and lacZ reporter gene induction with metal ions and chelators, amino acid analogs, antibiotics, inhibitors of oxidative phosphorylation and miscellaneous stress factors --
Nucleotide metabolism --
Utilization of small cartoon-containing molecules by Bacillus subtilis --
Screening for genes involved in the metabolism of small cartoon-containing molecules --
Nitrogen sources --
Induction or repression by nitrogen sources --
Glucose regulation in Bacillus subtilis --
Catabolite repression by glucose --
Cell processes and macromolecule metabolism: a method for the selection of mutants in genetic competence, DNA recombination and repair and protein secretion --
Molecular tools for the identification of mutations affecting protein secretion --
Screening for mutants defective in secretion --
Genes affecting motility --
Differentiation of Bacillus subtilis --
Methods for rapid screening of properties: spore resistance and germination --
Genes affecting the cell envelope --
Analysis of cell envelope components --
Pedtidoglycan structure and structural determination in vegetative cells of Bacillus subtilis --
Cell cycle gene analysis --
Identification and analysis of cell cycle genes --
Endospore formation --
Isolation and characterization of genes affecting sporulation or the cell cycle --
Selection and characterization of genes affecting germination --
The oxidative stress response --
Screening for increased sensitivity or resistance and level of lacZ reporter gene expression in response to the oxidizing agents hydrogen peroxide and methy viologen --
The osmotic shock response --
Salt stress --
Phosphate regulation --
Screening for mutants affected in their response to phosphate --
The heat shock response --
The cold shock response --
pH-inducible genes --
Analysis of the mutant collection for defects in their temperature and pH shock response --
Screening for growth and lacZ reporter gene induction or repression under glucose and phosphate starvation and under heat, oxidative and osmotic stress --
List of Bacillus subtilis unknown genes with a phenotype determined by the functional analysis project --
List of Bacillus subtilis genes of known function.
Other Titles: Bacterial genes
Responsibility: edited by Wolfgang Schumann, S. Dusko Ehrlich, Naotake Ogasawara.

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