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Functional analysis of DNA and chromatin

Author: Juan C Stockert; Jesús Espada; Alfonso Blázquez-Castro
Publisher: New York : Humana Press : Springer, ©2014.
Series: Methods in molecular biology (Clifton, N.J.), v. 1094.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

Covering standard and recently developed methods in functional analysis of genetic material, this new volume in the Methods in Molecular Biology series includes reproducible protocols, expert  Read more...

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Genre/Form: Laboratory Manuals
Laboratory manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Juan C Stockert; Jesús Espada; Alfonso Blázquez-Castro
ISBN: 9781627037051 1627037055 9781627037068 1627037063
OCLC Number: 857964155
Description: xv, 365 pages : illustrations ; 26 cm.
Contents: Predictive binding geometry of ligands to DNA minor groove : isohelicity and hydrogen-bonding pattern / Juan C. Stockert --
Using microchip gel electrophoresis to probe DNA-drug binding interactions / Nan Shi and Victor M. Ugaz --
Identifying different types of chromatin using Giemsa staining / Juan C. Stockert, Alfonso Blázquez-Castro, and Richard W. Horobin --
Analysis of DNA damage and repair by comet fluorescence in situ hybridization (comet-FISH) / Michael Glei and Wiebke Schlörmann --
Alkaline nuclear dispersion assays for the determination of DNA damage at the single cell level / Piero Sestili and Carmela Fimognari --
Polarization microscopy of extended chromatin fibers / Maria Luiza S. Mello and Benedicto de Campos Vidal --
DNA labeling in vivo : quantification of epidermal stem cell chromatin content in whole mouse hair follicles using Fiji image processing software / Elisa Carrasco, María I. Calvo, and Jesús Espada --
Historical overview of bromo-substituted DNA and sister chromatid differentiation / Roberto Mezzanotte and Mariella Nieddu --
Image analysis of chromatin remodelling / Benedicto de Campos Vidal, Marina B. Felisbino, and Maria Luiza S. Mello --
FISH methods in cytogenetic studies / Miguel Pita [and others] --
Ultrastructural and immunofluorescent methods for the study of the XY body as a biomarker / Roberta B. Sciurano and Alberto J. Solari --
Atomic force microscopy for analyzing metaphase chromosomes : comparison of AFM images with fluorescence labeling images of banding patterns / Osamu Hoshi and Tatsuo Ushiki --
Selective detection of phagocytic phase of apoptosis in fixed tissue sections / Vladimir V. Didenko --
Selective transport of cationized fluorescent topoisomerase into nuclei of live cells for DNA damage studies / Candace L. Minchew and Vladimir V. Didenko --
Visualization and interpretation of eukaryotic DNA replication intermediates in vivo by electron microscopy / Kai J. Neelsen [and others] --
Combined bidimensional electrophoresis and electron microscopy to study specific plasmid DNA replication intermediates in human cells / Cindy Follonier and Massimo Lopes --
Standard DNA methylation analysis in mouse epidermis : bisulfite sequencing, methylation-specific PCR, and 5-methyl-cytosine (5mC) immunological detection / Jesús Espada, Elisa Carrasco, and María I. Calvo --
Methyl-combing : single-molecule analysis of DNA methylation on stretched DNA fibers / Attila Németh --
Investigating 5-hydroxymethylcytosine (5hmC) : the state of the art / Colm E. Nestor [and others] --
Hydroxymethylated DNA immunoprecipitation (hmeDIP) / Colm E. Nestor and Richard R. Meehan --
Microscale thermophoresis for the assessment of nuclear protein-binding affinities / Wei Zhang [and others] --
Analysis of histone posttranslational modifications from nucleolus-associated chromatin by mass spectrometry / Stefan Dillinger [and others] --
Salt-urea, sulfopropyl-sepharose, and covalent chromatography methods for histone isolation and fractionation / Pedro Rodriguez-Collazo [and others] --
Chromatin immunoprecipitation / Javier Rodríguez-Ubreva and Esteban Ballestar --
Analysis of chromatin composition of repetitive sequences : the ChIP-Chop assay / Raffaella Santoro --
Purification of specific chromatin domains from single- copy gene loci in Saccharomyces cerevisiae / Stephan Hamperl [and others] --
Deep sequencing of small chromatin-associated RNA : isolation and library preparation / Sarah Daniela Diermeier, Thomas Schubert, and Gernot Längst --
Deep sequencing of small chromatin-associated RNA : bioinformatic analysis / Sarah Daniela Diermeier and Gernot Längst.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1094.; Springer protocols (Series)
Responsibility: edited by Juan C. Stockert, Jesús Espada, Alfonso Blázquez-Castro.

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