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Gene and chromosome analysis. Part C

Author: Kenneth W Adolph
Publisher: San Diego : Academic Press, ©1994.
Series: Methods in molecular genetics, v. 5.
Edition/Format:   Print book : EnglishView all editions and formats
Database:WorldCat
Summary:

A practical laboratory guide to current techniques of molecular biology and genetics. The focus of this volume is on eukaryotic cells, particularly human cells and other eukaryotic model systems.  Read more...

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Additional Physical Format: Online version:
Gene and chromosome analysis. Part C.
San Diego : Academic Press, ©1994
(OCoLC)646184983
Document Type: Book
All Authors / Contributors: Kenneth W Adolph
ISBN: 0120443074 9780120443079
OCLC Number: 31429065
Description: xv, 402 pages : illustrations ; 24 cm.
Contents: Part 1 Gene analysis: method of differential display; analysis of active genes; transcriptional regulation of gene expression and characterization of gene promoters and transcription factors; in vivo protein-DNA interactions associated with gene transcription; analysis of T-Cell receptor transcripts by the nonpalindromic adaptor polymerase chain reaction; preparation and use of nuclear extracts from Drosophila cells for in vitro splicing. Part 2 Recombinant proteins: antibody-plasminogen activator conjugates and recombinant proteins; baculovirus-mediated overexpression of the glucocorticoid and mineralocorticoid receptors and related proteins; gene amplification strategies for protein production in mammalian cells. Part 3 Chromosome analysis: manipulation of large genomic constructs using yeast artificial chromosomes; the analysis of disease-specific chromosomal rearrangements with microcloned DNA markers; somatic cell genetic methods in human chromosome mapping; nonisotopic in situ hybridization in paraffin-embedded tissue sections. Part 4 Chromosomal proteins and DNA: biochemical reconstitution of chromatin with physiological nucleosome spacing; analysis of telomerase and telomeres; isolation, purification, and analysis of specific gene-containing nucleoproteins and nucleoprotein complexes; detection and identification of proteins which recognize DNA damage. Part 5 Disease and development: structural and functional analysis of tumour suppressor p53; SOS function tests for studies of chemical carcinogenesis using Salmonella typhimurium; analysis of plasmid DNA after transfer into mammalian tissues; application of mouse crosses toward defining the genetics of disease phenotypes; multiple strategies for isolating mouse homologues of developmental control genes of Drosophila.
Series Title: Methods in molecular genetics, v. 5.
Responsibility: edited by Kenneth W. Adolph.

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