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Gene probes : principles and protocols

Author: Marilena Aquino de Muro; Ralph Rapley
Publisher: Totowa, NJ : Humana Press, ©2002.
Series: Methods in molecular biology (Clifton, N.J.), v. 179.
Edition/Format:   Print book : EnglishView all editions and formats
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Senior scientists Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using genes probes in a wide variety of  Read more...

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Genre/Form: Laboratory manuals
Manuels de laboratoire
laboratory manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Marilena Aquino de Muro; Ralph Rapley
ISBN: 0896038858 9780896038851
OCLC Number: 46504587
Description: xi, 272 pages : illustrations (some color) ; 24 cm.
Contents: Target format and hybridization conditions --
End labeling procedures: an overview --
Photobiotin labeling --
Nick translation --
Random primed labeling --
Design and evaluation of 16S rRNA-targeted oligonucleotide probes for fluorescence In Situ hybridization --
Evaluation of alternations in the tumor suppressor genes INK4A and INK4B in human bladder tumors --
The [beta]-tubulin gene region as a molecular marker to distinguish Leishmania parasites --
Detection of malignant plasma cells in the bone marrow and peripheral blood of patients with multiple myeloma --
Detection of Chlamydia trachomatis by DNA amplification --
Isolation and identification of Campylobacter species by use of selective enrichment, nucleic acid amplification methods, and gene probes --
Diagnosis of human papillomavirus using In Situ hybridization and In Situ polymerase chain reaction --
Quantitation of In Situ hybridization using image analysis of radioactively labeled RNA probes --
Stable isotope probing for detection of methanotrophs after enrichment with ¹³CH₄ --
Detection of Enterococci in freshwater and seawater (16S and 23S rRNA Enterococci Oligonucleotide Probes) --
Analysis of 16S-23S rRNA intergenic spacer of Vibrio cholerae and Vibrio mimicus for detection of these species --
Colony hybridization of bacterial isolates with Burkholderia cepacia-specific probes --
In Situ hybridization of Burkholderia cepacia cells --
Detection of polychlorinated biphenyl-degrading organisms in soil --
Detection of single-copy genes in DNA from transgenic plants --
Differentiation between transcripts of genes belonging to small families during fruit ripening and abscission --
Polymerase chain reaction detection of nvasive Shigella and Salmonella enterica in food --
Polymerase chain reaction for detection of Listeria monocytogenes.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 179.
Responsibility: edited by Marilena Aquino de Muro and Ralph Rapley.
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"In this book, the editors have brought together a comprehensive collection of time-tested protocols for designing and using gene probes in a wide variety of applications." -Clinical Laboratory Read more...

 
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