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Genetic Interactions Among Microorganisms in the Natural Environment.

Author: E M H Wellington; J D Van Elsas
Publisher: Burlington : Elsevier Science, 1992.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This book gives an overview of gene transfer and stability in those aquatic and terrestrial environments where bacteria and fungi can survive and interact genetically. It examines the role played by sex between microbes in the evolution of populations and their survival. Special emphasis is placed on methodology, including the analysis by novel techniques of genetic material extracted directly from soils, rivers and  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Wellington, E.M.H.
Genetic Interactions Among Microorganisms in the Natural Environment.
Burlington : Elsevier Science, ©1992
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: E M H Wellington; J D Van Elsas
ISBN: 9781483287775 1483287777
OCLC Number: 893740394
Notes: Chapter 9. Detection systems for phylloplane pseudomonads.
Description: 1 online resource (314 pages)
Contents: Front Cover; Genetic Interactions among Microorganisms in the Natural Environment; Copyright Page; Table of Contents; Contributors; Preface; Part I: General introduction; Chapter 1. The role of gene transfer in bacterial evolution; 1.1. The scope of this chapter; 1.2. The mechanisms of bacterial gene transfer; 1.3. The consequences of bacterial gene transfer; 1.4. What can we conclude?; References; Part II: Evidence for gene transfer between bacteria in nature; Chapter 2. Antibiotic resistance gene transfer in the environment: an overview; 2.1. Introduction. 2.2. Antibiotic resistance mechanisms and genes2.3. Indirect (retrospective) evidence of natural antibiotic resistance gene transfer; 2.4. Scope of natural genes transfer mechanisms; 2.5. Environments favouring antibiotic resistance gene transfer; 2.6. Concluding remarks; Acknowledgements; References; Part III: Methods for studying genetic interactions in terrestrial environments; Chapter 3. Horizontal gene transfer in bacterial soil communities and its significance for risk assessment; 3.1. Introduction; 3.2. Evidence for distribution of microbial genes in natural environments. 3.3. Widespread class II transposons contain systems for gene integration and dispersal among bacteria3.4. In situ horizontal mobility and intra-species expression of rDNA; 3.5. Is a pool of free DNA available for soil microorganisms in situ!; 3.6. Is genetic communication assisted by soil animals?; 3.7. Problems of evaluating gene mobility for risk assessment analysis; 3.8. Conclusions; Acknowledgements; References; Chapter 4. Detection of genetic exchange in the terrestrial environment; 4.1. Introduction; 4.2. Sterile soil systems; 4.3. Gene transfer in non-sterile soil. 4.4. The direct extraction of DNA and RNA from soil for analysis of inoculant and excipient populations4.5. General considerations about sex in the soil bacterium Streptomyces; 4.6. General conclusions; Acknowledgments; References; Chapter 5. Detection systems for streptomycetes; 5.1. Introduction; 5.2. Immunological detection; 5.3. Development and detection of a genetic marker system; 5.4. Summary and conclusions; Acknowledgements; References; Chapter 6. Extraction of Streptomyces spores from soil and detection of rare gene transfer events; 6.1. Introduction. 6.2. Method development and experimental resultsAcknowledgements; References; Chapter 7. Methods for extracting bacterial cells from soil; 7.1. Introduction; 7.2. Methodological development; 7.3. Discussion of results; 7.4. Conclusions; References; Chapter 8. Methods for studying conjugative plasmid transfer in soil; 8.1. Introduction; 8.2. Plating methodology; 8.3. Transconjugant isolation and enumeration; 8.4. Selectable markers; 8.5. Genetic elements; 8.6. Colony hybridisation; 8.7. Donor counterselection; 8.8. Plasmid transfer to indigenous soil bacteria; Acknowledgement; References.

Abstract:

This book gives an overview of gene transfer and stability in those aquatic and terrestrial environments where bacteria and fungi can survive and interact genetically. It examines the role played by sex between microbes in the evolution of populations and their survival. Special emphasis is placed on methodology, including the analysis by novel techniques of genetic material extracted directly from soils, rivers and lakes. The natural spread of antibiotic resistance and the safe use of genetically manipulated microbes are matters of considerable scientific, medical and public concern upon whic.

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