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Genomics protocols

Author: Michael P Starkey; Ramnath Elaswarapu
Publisher: Totowa, N.J. : Humana Press, ©2001.
Series: Methods in molecular biology (Clifton, N.J.), v. 175.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Genome analysis is essential both to understanding the molecular bases of physiological processes and to the development of novel therapies for treating human diseases. In Genomics Protocols, an expert panel of internationally acclaimed researchers from academia, industry, and leading genome centers provides a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Manuels de laboratoire
Additional Physical Format: Print version:
Genomics protocols.
Totowa, N.J. : Humana Press, ©2001
(DLC) 00046106
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Michael P Starkey; Ramnath Elaswarapu
ISBN: 0896037746 9780896037748 0585404372 9780585404370 159259235X 9781592592357
OCLC Number: 50175102
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xiv, 538 pages) : illustrations.
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: 1. Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse / Paul A. Lyons --
2. Genetic Analysis of Complex Traits / Stephen P. Bryant and Mathias N. Chiano --
3. Sequence-Based Detection of Single Nucleotide Polymorphisms / Deborah A. Nickerson, Natali Kolker, Scott L. Taylor and Mark J. Rieder --
4. Genomic Mismatch Scanning for the Mapping of Genetic Traits / Farideh Mirzayans and Michael A. Walter --
5. Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization / Mario A.J.A. Hermsen, Marjan M. Weiss, Gerrit A. Meijer and Jan P.A. Baak --
6. Construction of a Bacterial Artificial Chromosome Library / Sangdun Choi and Ung-Jin Kim --
7. Contiguation of Bacterial Clones / Sean J. Humphray, Susan J. Knaggs and loannis Ragoussis --
8. Mapping of Genomic Clones by Fluorescence In Situ Hybridization / Margaret A. Leversha --
9. Map Integration : From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map / Panagiotis Deloukas --
10. Construction of Full-Lengin-tnriched cDNA Libraries : The Oligo-Capping Method / Yutaka Suzuki and Sumio Sugano --
11. Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping : The Human Gene Map / Panagiotis Deloukas --
12. Preparation and Screening of High-Density cDNA Arrays with Genomic Clones / Gunther Zehetner, Maria Pack, and Katja Schafer --
13. Direct Selection of cDNAs by Genomic Clones / Daniela Toniolo --
14. Exon Trapping : Application of a Large-Insert Multiple-Exon-Trapping System / Martin C. Wapenaar and Johan T. Den Dunnen --
15. Sequencing Bacterial Artificial Chromosomes / David E. Harris and Lee Murphy --
16. Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics / Yvonne J.K. Edwards and Simon M. Brocklehurst --
17. Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning / Greg Elgar --
18. Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization / Oliver Dorian von Stein --
19. Isolation of Differentially Expressed Genes by Representational Difference Analysis / Christine Wallrapp and Thomas M. Gress --
20. Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display / Michael P. Starkey --
21. Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos / Nicolas Pollet and Christof Niehrs --
22. Expression Monitoring Using cDNA Microarrays : A General Protocol / Xing Jian Lou, Mark Schena, Frank T. Horrigan, Richard M. Lawn and Ronald W. Davis --
23. Prediction of Protein Structure and Function by Using Bioinformatics / Yvonne J.K. Edwards and Amanda Cottage --
24. Identification of Novel Genes by Gene Trap Mutagenesis / Anne K. Voss and Tim Thomas --
25. Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice / Ahmed Mansouri --
26. Gehomic Analysis Utilizing the Yeast Two-Hybrid System / llya G. Serebriiskii, Garabet G. Toby, Russell L. Finley, Jr. and Erica A. Golemis --
27. Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle / Terry J. Amiss and Richard Jude Samulski --
28. Retroviral-Mediated Gene Transduction / Donald S. Anson --
29. Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods / Ruth Gjerset, All Haghighi, Svetlana Lebedeva, and Dan Mercola --
30. Ribozyme Gene Therapy / Leonidas A. Phylactou.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 175.
Responsibility: edited by Michael P. Starkey and Ramnath Elaswarapu.

Abstract:

The technologies described extend from those traditionally recognized as coming under the genomics umbrella, touch on proteomics (the study of the expressed protein complement of the genome), through  Read more...

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"The two editors of this volume have invited contributors from a variety of authors to write a laboratory manual dealing exclusively with the application of genomic methods in eukaryotes....Each Read more...

 
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