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Germline stem cells : methods and protocols

Author: Michael Buszczak
Publisher: New York : Humana Press : Springer, ©2017.
Series: Methods in molecular biology (Clifton, N.J.), v. 1463.; Springer protocols (Series)
Edition/Format:   eBook : Document : English : 2nd editionView all editions and formats
Summary:
This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter's guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Cell microencapsulation.
New York : Humana Press, ©2017
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Michael Buszczak
ISBN: 9781493940172 1493940171
OCLC Number: 960723405
Description: 1 online resource (xi, 229 pages) : illustrations (chiefly color).
Contents: Analysis of the C. elegans germline stem cell pool / Sarah L. Crittenden, Hannah S. Seidel, and Judith Kimble --
Methods for studying the germline of the human parasite Schistosoma mansoni / Julie N.R. Collins and James J. Collins III --
Evaluation of the asymmetric division of Drosophila male germline stem cells / Mayu Inaba and Yukiko M. Yamashita --
Live imaging of the Drosophila testis stem cell niche / Leah J. Greenspan and Erika L. Matunis --
RNA isolation from early Drosophila larval ovaries / Dana Gancz and Lilach Gilboa --
Live-cell imaging of the adult Drosophila ovary using confocal microscopy / Nevine A. Shalaby and Michael Buszczak --
Measurement of mRNA Poly(A) tail lengths in Drosophila female germ cells and germ-line stem cells / Aymeric Chartier, Willy Joly, and Martine Simonelig --
Identification of germ-line stem cells in zebrafish / Bruce W. Draper --
Primordial germ cell isolation from Xenopus laevis embryos / Amanda M. Butler [and others] --
Single-cell lineage analysis of oogenesis in mice / Lei Lei and Allan C. Spradling --
Visualization and lineage tracing of Pax7+ spermatogonial stem cells in the mouse / Gina M. Aloisio [and others] --
Transplantation as a quantitative assay to study mammalian male germline stem cells / Aileen R. Helsel and Jon M. Oatley --
Mouse fetal germ cell isolation and culture techniques / Cassy M. Spiller, Guillaume Burnet, and Josephine Bowles --
Rattus norvegicus spermatogenesis colony-forming assays / F. Kent Hamra --
Identification of mouse piRNA pathway components using anti-MIWI2 antibodies / Takamasa Hirano, Hidetoshi Hasuwa, and Haruhiko Siomi --
Efficient induction and isolation of human primordial germ cell-like cells from competent human pluripotent stem cells / Naoko Irie and M. Azim Surani.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1463.; Springer protocols (Series)
Responsibility: edited by Michael Buszczak.

Abstract:

This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter's guide readers through methods and principles on primordial  Read more...

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