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Glycosyltransferases : methods and protocols

Autor: Inka Brockhausen
Editora: New York : Humana Press, ©2013.
Séries: Methods in molecular biology (Clifton, N.J.), 1022.
Edição/Formato   e-book : Documento : InglêsVer todas as edições e formatos
Base de Dados:WorldCat
Resumo:

This book contains a wide range of studies, methods and protocols which form a solid basis for investigations of the role and mechanisms, biology and pathology involving glycosyltransferases (GTs).  Ler mais...

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Detalhes

Gênero/Forma: Laboratory Manuals
Electronic books
Laboratory manuals
Tipo de Material: Documento, Recurso Internet
Tipo de Documento: Recurso Internet, Arquivo de Computador
Todos os Autores / Contribuintes: Inka Brockhausen
ISBN: 9781627034654 162703465X
Número OCLC: 850907340
Descrição: 1 online resource (xvii, 425 pages) : illustrations.
Conteúdos: Glycan microarray screening assay for glycosyltransferase specificities / W. Peng, C.M. Nycholat and N. Razi --
A fluorescence-based assay for core 1 [beta]3galactosyltransferase (T-synthase) activity / T. Ju and R.D. Cummings --
Structural and biochemical analysis of a bacterial glycosyltransferase / F. Zhu [and others] --
Study of the biological functions of mucin type core 3 O-glycans / S.H. Lee and M. Fukuda --
Generation of anti-sulfated glycan antibodies using sulfotransferase-deficient mice / H. Kawashima --
Fluorescent microscopy as a tool to elucidate dysfunction and mislocalization of golgi glycosyltransferases in COG complex depleted mammalian cells / R.A. Willett, I.D. Pokrovskaya and V.V. Lupashin --
A practical approach to reconstruct evolutionary history of animal sialyltransferases and gain insights into the sequence-function relationships of golgi-glycosyltransferases / D. Petit [and others] --
Fluorescent lectin staining of drosophila embryos and tissues to detect the spatial distribution of glycans during development / E. Tian, L. Zhang and K.G. Ten Hagen --
Photoaffinity labeling of protein O-mannosyltransferases of the PMT1/PMT2 subfamily / M. Loibl and S. Strahl --
Enzymatic analysis of the protein O-glycosyltransferase, rumi, acting toward epidermal growth factor-like (egf) repeats / H. Takeuchi and R.S. Haltiwanger --
Enzymatic characterization of recombinant enzymes of O-glcNAc cycling / E.J. Kim and J.A. Hanover --
Antibodies and activity measurements for the detection of o-glcnac transferase and assay of its substrate, UDP-glcNAc / T. Lefebvre [and others] --
In vitro glycosylation assay for bacterial oligosaccharyltransferases / M.A. Musumeci, M.V. Ielmini and M.F. Feldman --
In vitro UDP-sugar:Undecaprenyl-phosphate sugar-1-phosphate transferase assay and product detection by thin layer chromatography / K.B. Patel and M.A. Valvano --
In vitro O-antigen ligase assay / X. Ruan and M.A. Valvano --
Functional identification of bacterial glucosyltransferase WbdN / Y. Gao, A. Vinnikova and I. Brockhausen --
Methods for the pasteurella glycosaminoglycan synthases: Enzymes that polymerize hyaluronan, chondroitin, or heparosan chains / P.L. Deangelis --
Methods for measuring class i membrane-bound hyaluronan synthase activity / P.H. Weigel, A.J. Padgett-McCue and B.A. Baggenstoss --
Creation and characterization of glycosyltransferase mutants of Trypanosoma brucei / L. Izquierdo, M.L. Guther and M.A. Ferguson --
Mannose-6-phosphate: A regulator of LLO destruction / N. Gao and M.A. Lehrman --
N-acetylglucosaminyltransferase (GnT) assays using fluorescent oligosaccharide acceptor substrates: GnT-III, IV, V, and IX (GnT-Vb) / S. Takamatsu [and others] --
A method for determination of UDP-GlcNAc: GlcNAc[beta]1-6(GlcNAc[beta]1-2)man[alpha]1-r [GlcNAc to man] beta1-4n-acetylglucosaminyltransferase vi activity / T. Taguchi and N. Taniguchi --
In vitro assays of orphan glycosyltransferases and their application to identify notch xylosyltransferases / M.K. Sethi [and others] --
In vitro folding of [beta]-1,4galactosyltransferase and polypeptide-[alpha]-N-acetylgalactosaminyltransferase from the inclusion bodies / B. Ramakrishnan and P.K. Qasba --
An assay for [alpha] 1,6-fucosyltransferase (FUT8) activity based on the HPLC separation of a reaction product with fluorescence detection / H. Ihara [and others] --
Immunodetection of glycosyltransferases in gastrointestinal tissues / J. Gomes and C.A. Reis --
Glycosyltransferases in chemo-enzymatic synthesis of oligosaccharides / B. Tefsen and I. van Die --
Tumor targeting by a carbohydrate ligand-mimicking peptide / S. Hatakeyama [and others] --
Glycoengineering of human cell lines using zinc finger nuclease gene targeting: Simple cells with homogeneous GalNAc O-glycosylation allow isolation of the O-glycoproteome by one-step lectin affinity chromatography / C. Steentoft, E.P. Bennett and H. Clausen --
Generation of monoclonal antibodies to native active human glycosyltransferases / M.B. Vester-Christensen [and others].
Título da Série: Methods in molecular biology (Clifton, N.J.), 1022.
Responsabilidade: edited by Inka Brockhausen.
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