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Gnotobiotic mouse technology : an illustrated guide

Author: Chriss J Vowles; Natalie E Anderson; Kathryn A Eaton
Publisher: Boca Raton : CRC Press, Taylor & Francis Group, [2016]
Edition/Format:   Print book : EnglishView all editions and formats
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Genre/Form: Laboratory Manuals
Laboratory manuals
Document Type: Book
All Authors / Contributors: Chriss J Vowles; Natalie E Anderson; Kathryn A Eaton
ISBN: 9781498736329 1498736327
OCLC Number: 931794813
Notes: Includes index.
Description: xvi, 237 pages : color illustrations ; 24 cm
Contents: 1. Brief history of germ-free life --
2 . Overview of gnotobiotic technology --
3. Equipment and terminology --
4. Personal protective equipment (PPE) --
5. Sterilants and sterilization --
6. Isolator setup --
7. Port entry and exit --
8. Sterilizing food and supplies --
9. Supply cylinder entry --
10. Isolator maintenance --
11. Aseptic mouse transfer --
12. Working with germ-free or gnotobiotic mice in the class II biosafety cabinet --
13. Shipping mice --
14. Rederivation --
15. Microbiological testing --
16. Genetic testing --
17. Facility setup and management.
Responsibility: Chriss J. Vowles, Natalie E. Anderson, Kathryn A. Eaton.

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"This book introduces and describes techniques utilized for the production, maintenance, and experimental manipulation of germ-free and gnotobiotic mice in a research support facility. The Read more...

 
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