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Guide to yeast genetics : functional genomics, proteomics, and other systems analysis

Author: Jonathan Weissman; Christine Guthrie; Gerald R Fink
Publisher: Amsterdam ; Boston : Elsevier/Academic, 2010.
Series: Methods in enzymology, v. 470.
Edition/Format:   Print book : English : 2nd edView all editions and formats
Database:WorldCat
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Part of the "Methods in Enzymology" series, this guide to yeast genetics and molecular cell biology is intends to meet the needs of graduate students, postdoctoral students, and researchers by  Read more...

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Document Type: Book
All Authors / Contributors: Jonathan Weissman; Christine Guthrie; Gerald R Fink
ISBN: 9780123751720 0123751721 9780123751713 0123751713
OCLC Number: 477255008
Description: lii, 892 pages, [16] pages of plates : illustrations (some color) ; 24 cm.
Contents: Section I. Functional genomics --
1. Analysis of gene function using DNA microarrays / Andrew P. Capaldi --
2. An introduction to microarray data analysis and visualization / Gregg B. Whitworth --
3. Genome-wide approaches to monitor pre-mRNA splicing / Maki Inada and Jeffrey A. Pleiss --
4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites / Philippe Lefrançois, Wei Zheng and Michael Snyder --
5. Genome-wide mapping of nucleosomes in yeast / Oliver J. Rando --
6. Genome-wide translational profiling by ribosome footprinting / Nicholas T. Ingolia --
Section II. Systematic genetic analysis --
7. Synthetic genetic array (SGA) analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe / Anastasia Baryshnikova [and others] --
8. Making temperature-sensitive mutants / Shay Ben-Aroya [and others] --
9. Quantitative genetic interaction mapping using the E-MAP approach / Sean R. Collins, Assen Roguev and Nevan J. Krogan --
10. Exploring gene function and drug action using chemogenomic dosage assays / Elke Ericson [and others] --
Section III. Proteomics --
11. Yeast expression proteomics by high-resolution mass spectrometry / Tobias C. Walther, Jesper V. Olsen and Matthias Mann --
12. High-quality binary interactome mapping / Matija Dreze [and others] --
13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry-based proteomics / Bernd Bodenmiller and Ruedi Aebersold --
14. A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells / Stephen W. Michnick [and others] --
15. Yeast lipid analysis and quantification by mass spectrometry / Xue Li Guan [and others] --
16. Mass spectrometry-based metabolomics of yeast / Christopher A. Crutchfield [and others] --
Section IV. Systems Analysis --
17. Imaging single mRNA molecules in yeast / Hyun Youk, Arjun Raj and Alexander van Oudenaarden --
18. Reconstructing gene histories in Ascomycota fungi / Ilan Wapinski and Aviv Regev --
19. Experimental evolution in yeast: a practical guide / Maitreya J. Dunham --
20. Enhancing stress resistance and production phenotypes through transcriptome engineering / Felix H. Lam [and others] --
Section V. Advances in Cytology/Biochemistry --
21. Visualizing yeast chromosomes and nuclear architecture / Peter Meister [and others] --
22. Quantitative localization of chromosomal loci by immunofluorescence / Donna Garvey Brickner, William Light and Jason H. Brickner --
23. Spinning disk confocal microscopy of yeast / Kurt Thorn --
24. Correlative GFP-immunoelectron microscopy in yeast / Christopher Buser and Kent McDonald --
25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae / J. Ross Buchan, Tracy Nissan and Roy Parker --
26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization / Daniel Zenklusen and Robert H Singer --
27. The Use of in vitro assays to measure endoplasmic reticulum-associated degradation (ERAD) / Jeffrey L. Brodsky --
28. A protein transformation protocol for introducing yeast prion particles into yeast / Motomasa Tanaka --
29. Overexpression and purification of integral membrane proteins in yeast / Franklin A. Hays, Zygy Roe-Zurz, and Robert M. Stroud --
30. Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast / Simon Alberti, Randal Halfmann and Susan Lindquist --
Part VI. Other fungi --
31. Genetics and molecular biology in Candida albicans / Aaron D. Hernday [and others] --
32. Molecular genetics of Schizosaccharomyces pombe / Sarah A. Sabatinos and Susan L. Forsburg --
33. Applying genetics and molecular biology to the study of the human pathogen Cryptococcus neoformans / Cheryl D. Chun and Hiten D. Madhani --
34. The Fungal Genome Initiative and lessons learned from genome sequencing / Christina A. Cuomo and Bruce W. Birren --
35. Ultradian metabolic cycles in yeast / Benjamin P. Tu.
Series Title: Methods in enzymology, v. 470.
Responsibility: edited by Jonathan Weissman, Christine Guthrie, Gerald R. Fink.

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Praise for the first edition: "...this is an excellent collection of methods that is certain to be an important handbook for yeast researchers."--Will Prinz, National Institutes of Health for Read more...

 
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