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Handbook of methods in aquatic microbial ecology

Author: P F Kemp
Publisher: Boca Raton : Lewis Publishers, ©1993.
Edition/Format:   Print book : EnglishView all editions and formats
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Presents a comprehensive compilation of 85 fundamental methods in modern aquatic microbial ecology. This book also presents practical advice on how to apply the methods. It is suitable for marine and  Read more...

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Genre/Form: Handbooks and manuals
Handbooks, manuals, etc
Guides, manuels, etc
Named Person: Wasser
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: P F Kemp
ISBN: 0873715640 9780873715645
OCLC Number: 27768277
Description: 777 pages : illustrations ; 27 cm
Contents: Isolation and enumeration of anaerobic and microaerophilic bacteria in aquatic habitats / by M.J. Ferrara-Guerrero, D.G. Marty, and A. Bianchi --
Isolation and cultivation of hyperthermophilic bacteria from marine and freshwater habitats / John A. Baross --
Isolation of psychrophilic bacteria / Richard Y. Morita --
Isolation and characterization of bacteriocytes from a bivalve-sulfur bacterium symbiosis / Steven C. Hand and Amy E. Anderson --
General techniques for the isolation and culture of marine protists from estuarine, littoral, psammolittoral, and sublittoral waters / John J. Lee --
Long-term culture of marine benthic protists / Philip G. Carey --
Behavior and bioenergetics of anaerobic and microaerobic protists / B.J. Finlay --
Culturing free-living marine phagotrophic dinoflagellates / Evelyn J. Lessard --
Enrichment, isolation, and culture of free-living heterotrophic flagellates / David A. Caron --
Determination of pressure effects on flagellates isolated from surface waters / C.M. Turley --
Isolation, cloning, and axenic cultivation of marine ciliates / A.T. Soldo and S.A. Brickson --
Isolation and laboratory culture of marine oligotrichous ciliates / Dian J. Gifford --
Extraction of protists in aquatic sediments via density gradient centrifugation / Daniel M. Alongi --
Statistical analysis of direct counts of microbial abundance / David L. Kirchman --
Enumeration and isolation of viruses / Curtis A. Suttle --
Total count of viruses in aquatic environments / Gunnar Bratbak and Mikal Heldal --
Improved sample preparation for enumeration of aggregated aquatic substrate bacteria / M. Iqubal Velji and Lawrence J. Albright --
Direct estimates of bacterial numbers in seawater samples without incurring cell loss due to sample storage / C.M. Turley --
Total and specific bacterial counts by simultaneous staining with DAPI and fluorochrome-labeled antibodies / Kjell Arne Hoff --
Use of RFLPs for the comparison of marine cyanobacteria / Susan E. Douglas --
Use of high-resolution flow cytometry to determine the activity and distribution of aquatic bacteria / D.K. Button and B.R. Robertson --
Phytoplankton analysis using flow cytometry / Robert J. Olson, Erik R. Zettler, and Michele D. DuRand --
Enumeration of phototrophic picoplankton by autofluorescence microscopy / Erland A. Maclsaac and John G. Stockner --
Estimating cell concentration and biomass of autotrophic plankton using microscopy / Beatrice C. Booth --
Preservation and storage of samples for enumeration of heterotrophic protists / Evelyn B. Sherr and Barry F. Sherr --
Staining of heterotrophic protists for visualization via epifluorescence microscopy / Evelyn B. Sherr, David A. Caron, and Barry F. Sherr --
A quantitative protargol stain (QPS) for ciliates and other protists / D.J.S. Montagnes and D.H. Lynn --
Preparation of pelagic protists for electron microscopy / B.S.C. Leadbeater --
A rapid technique for the taxonomy of methanogenic bacteria : comparison of the methylreductase subunits / Pierre E. Rouviere and Carla H. Kuhner --
Extraction of DNA from soils and sediments / Ronald M. Atlas --
Detecting gene sequences using the polymerase chain reaction / Ronald M. Atlas --
Quantitative description of microbial communities using lipid analysis / Robert H. Findlay and Fred C. Dobbs --
Single-cell identification using fluorescently labeled, ribosomal RNA-specific probes / Edward F. DeLong --
Immunofluorescence method for the detection and characterization of marine microbes / Lisa Campbell --
The relationship between biomass and volume of bacteria / Svein Norland --
Microscope methods for measuring bacterial biovolume : epifluorescence microscopy, scanning electron microscopy, and transmission electron microscopy / Gunnar Bratbak --
Measurement of carbon and nitrogen biomass and biovolume from naturally derived marine bacterioplankton / SangHoon Lee --
Use of color image analysis and epifluorescence mircoscopy to measure plankton biomass / Peter G. Verity and Michael E. Sieracki --
Determination of size and morphology of aquatic bacteria by automated image analysis / Roland Psenner --
Analysis of microbial lipids to determine biomass and detect the response of sedimentary microorganisms to disturbance / Fred C. Dobbs and Robert H. Findlay --
Total microbial biomass estimation derived from the measurement of particulate adenosine-5'-triphosphate / David M. Karl --
Microphytobenthic biomass measurement using HPLC and conventional pigment analysis / Catherine Riaux-Gobin and Bert Klein --
Microphotometric analysis of the spectral absorption and fluorescence of individual phytoplankton cells and detrital matter / Rodolfo Iturriaga and Susan L. Bower --
Measurement of elemental content and dry weight of single cells : x-raymicroanalysis / Mikal Heidal --
Microautoradiographic detection of microbial activity / Kevin R. Carman --
14C tracer method for measuring microbial activity in deep-sea sediments / Jody W. Deming --
Evaluating bacterial activity from cell-specific ribosomal RNA content measured with oligonucleotide probes / P.F. Kemp, S. Lee, and J. LaRoche --
Use of fluorogenic model substrates for extracellular enzyme activity (EEA) measurement of bacteria / Hans-Georg Hoppe --
Photoassimilation of acetate by algae / Russell L. Cuhel --
Starvation-survival strategies in bacteria / Richard Y. Morita --
Community respiration measurements using pulsed O2 electrode / Christopher Langdon --
Sediment community production and respiration measurements : the use of microelectrodes and bell jars / P.A.G. Hofman and S.A. de Jong --
Distinguishing bacterial from nonbacterial decomposition of spartina alterniflora by respirometry / David E. Padgett --
Microbial RNA and DNA synthesis derived from the assimilation of [2,3H]-adenine / David M. Karl --
Adenosine triphosphate (ATP) and total adenine nucleotide (TAN) pool turnover rates as measures of energy flux and specific growth rate in natural populations of microorganisms / David M. Karl --
Estimating production of heterotrophic bacterioplankton via incorporation of tritiated thymidine / Russell T. Bell --
Thymidine incorporation into DNA as an estimate of sediment bacterial production / Stuart Findlay --
Leucine incorporation as a measure of biomass production by heterotrophic bacteria / David L. Kirchman --
Estimating conversion factors for the thymidine and leucine methods for measuring bacterial production / David L. Kirchman and Hugh W. Ducklow --
Bacterial production in anerobic water columns / Carlos Pedros-Alio, Josefina Garcia-Cantizano, and Juan I. Calderon --
Production of heterotrophic bacteria inhabiting marine snow / Alice L. Alldredge --
Bacterial growth rates measured by pulse labeling / Paul LaRock and Jung-Ho Hyun --
Utilization of amino acids and precursors for amino acid de novo synthesis by planktonic bacteria / Meinhard Simon --
Dialysis bag incubation as a nonradiolabeling technique to estimate bacterioplankton production in situ / Gerhard J. Herndl, Elisabeth Kaltenbock, and Gerald Muller-Niklas --
Growth rates of natural populations of heterotrophic nanoplankton / George B. McManus --
The labeled chlorophyll a technique for determining photoautotrophic carbon specific growth rates and carbon biomass / Donald G. Redalje --
Incorporation of 14CO2 into protein as an estimate of phytoplankton N-assimilation and relative growth rate / Giacomo R. DiTullio --
Membrane-containing fungal mass and fungal specific growth rate in natural samples / Steven Y. Newell --
Radiotracer approaches for the study of plant polymer biodegradation / Ronald Benner --
Estimating degradation rates of chitin in aquatic samples / Michael T. Montgomery and David L. Kirchman --
Measurement of dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) in seawater and estimation of DMS turnover rates / Ronald P. Kiene --
Sulfate assimilation by aquatic microorganisms / Russell L. Cuhel --
Determination of nitrogenase activity in aquatic samples using the acetylene reduction procedure / Douglas G. Capone --
Denitrification and nitrification rates in aquatic sediments / Sybil P. Seitzinger --
Turnover of 15 NH4 + tracer in sediments / T.H. Blackburn --
Microbial cycling of inorganic and organic phosphorus in the water column / James W. Ammerman --
Approaches for measuring stable carbon and nitrogen isotopes in bacteria / Richard B. Coffin and Luis A. Cifuentes --
Bacterial sinking losses / Carlos Pedros-Alio and Jordi Mas --
Methods for the observation and use in feeding experiments of microbial exopolymers / Alan W. Decho --
Protistan grazing rates via uptake of fluorescently labeled prey / Evelyn B. Sherr and Barry F. Sherr --
Grazing rate of bacterioplankton via turnover of genetically marked minicells / Johan Wikner --
Estimating rates of growth and grazing mortality of phytoplankton by the dilution method / Michael R. Landry --
Consumption of protozoa by copepods feeding on natural microplankton assemblages / Dian J. Gifford --
Predation on planktonic protists assessed by immunochemical assays / Mark D. Ohman --
Absorption of microbes by benthic macrofauna by the 14C:51CR dual-labeling method / Glenn Lopez --
Radioisotope technique to quantify in situ microbivory by meiofauna in sediments / Paul A. Montagna.
Responsibility: edited by Paul F. Kemp [and others].
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Primary Entity

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Caron -- Determination of pressure effects on flagellates isolated from surface waters / C.M. Turley -- Isolation, cloning, and axenic cultivation of marine ciliates / A.T. Soldo and S.A. Brickson -- Isolation and laboratory culture of marine oligotrichous ciliates / Dian J. Gifford -- Extraction of protists in aquatic sediments via density gradient centrifugation / Daniel M. Alongi -- Statistical analysis of direct counts of microbial abundance / David L. Kirchman -- Enumeration and isolation of viruses / Curtis A. Suttle -- Total count of viruses in aquatic environments / Gunnar Bratbak and Mikal Heldal -- Improved sample preparation for enumeration of aggregated aquatic substrate bacteria / M. Iqubal Velji and Lawrence J. Albright -- Direct estimates of bacterial numbers in seawater samples without incurring cell loss due to sample storage / C.M. 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DeLong -- Immunofluorescence method for the detection and characterization of marine microbes / Lisa Campbell -- The relationship between biomass and volume of bacteria / Svein Norland -- Microscope methods for measuring bacterial biovolume : epifluorescence microscopy, scanning electron microscopy, and transmission electron microscopy / Gunnar Bratbak -- Measurement of carbon and nitrogen biomass and biovolume from naturally derived marine bacterioplankton / SangHoon Lee -- Use of color image analysis and epifluorescence mircoscopy to measure plankton biomass / Peter G. Verity and Michael E. Sieracki -- Determination of size and morphology of aquatic bacteria by automated image analysis / Roland Psenner -- Analysis of microbial lipids to determine biomass and detect the response of sedimentary microorganisms to disturbance / Fred C. Dobbs and Robert H. Findlay -- Total microbial biomass estimation derived from the measurement of particulate adenosine-5'-triphosphate / David M. Karl -- Microphytobenthic biomass measurement using HPLC and conventional pigment analysis / Catherine Riaux-Gobin and Bert Klein -- Microphotometric analysis of the spectral absorption and fluorescence of individual phytoplankton cells and detrital matter / Rodolfo Iturriaga and Susan L. Bower -- Measurement of elemental content and dry weight of single cells : x-raymicroanalysis / Mikal Heidal -- Microautoradiographic detection of microbial activity / Kevin R. Carman -- 14C tracer method for measuring microbial activity in deep-sea sediments / Jody W. Deming -- Evaluating bacterial activity from cell-specific ribosomal RNA content measured with oligonucleotide probes / P.F. Kemp, S. Lee, and J. LaRoche -- Use of fluorogenic model substrates for extracellular enzyme activity (EEA) measurement of bacteria / Hans-Georg Hoppe -- Photoassimilation of acetate by algae / Russell L. Cuhel -- Starvation-survival strategies in bacteria / Richard Y. 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Redalje -- Incorporation of 14CO2 into protein as an estimate of phytoplankton N-assimilation and relative growth rate / Giacomo R. DiTullio -- Membrane-containing fungal mass and fungal specific growth rate in natural samples / Steven Y. Newell -- Radiotracer approaches for the study of plant polymer biodegradation / Ronald Benner -- Estimating degradation rates of chitin in aquatic samples / Michael T. Montgomery and David L. Kirchman -- Measurement of dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) in seawater and estimation of DMS turnover rates / Ronald P. Kiene -- Sulfate assimilation by aquatic microorganisms / Russell L. Cuhel -- Determination of nitrogenase activity in aquatic samples using the acetylene reduction procedure / Douglas G. Capone -- Denitrification and nitrification rates in aquatic sediments / Sybil P. Seitzinger -- Turnover of 15 NH4 + tracer in sediments / T.H. Blackburn -- Microbial cycling of inorganic and organic phosphorus in the water column / James W. Ammerman -- Approaches for measuring stable carbon and nitrogen isotopes in bacteria / Richard B. Coffin and Luis A. Cifuentes -- Bacterial sinking losses / Carlos Pedros-Alio and Jordi Mas -- Methods for the observation and use in feeding experiments of microbial exopolymers / Alan W. Decho -- Protistan grazing rates via uptake of fluorescently labeled prey / Evelyn B. Sherr and Barry F. Sherr -- Grazing rate of bacterioplankton via turnover of genetically marked minicells / Johan Wikner -- Estimating rates of growth and grazing mortality of phytoplankton by the dilution method / Michael R. Landry -- Consumption of protozoa by copepods feeding on natural microplankton assemblages / Dian J. Gifford -- Predation on planktonic protists assessed by immunochemical assays / Mark D. 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