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Handbook of molecular and cellular methods in biology and medicine

Author: Peter B Kaufman
Publisher: Boca Raton : CRC Press, ©1995.
Edition/Format:   Print book : English
Summary:

Covers a range of current methods including some extensively tested techniques. Methods include: isolation and purification of DNA, RNA and proteins; preparation of hybridization probes; blotting  Read more...

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Genre/Form: Laboratory Manuals
Laboratory manuals
Document Type: Book
All Authors / Contributors: Peter B Kaufman
ISBN: 0849325110 9780849325113
OCLC Number: 30809263
Description: 484 pages : illustrations ; 26 cm
Contents: Isolation and purification of DNA --
Isolation and purification of RNA --
Extraction and purification of proteins/enzymes --
Preparation of nucleic acid probes --
Electrophoresis, blotting and hybridization --
cDNA libraries --
Genomic DNA libraries --
DNA sequencing --
PCR techniques and applications --
DNA fingerprinting --
DNA mutagenesis in vitro --
Inhibition of gene expression by antisense DNA and RNA --
DNA footprinting and gel retardation assay --
Translation of mRNA(s) and analysis of proteins by gel electrophoresis. Gene transfer and expression in animals --
Gene transfer and expression in plants --
Plant cell culture --
Plant tissue culture --
Microscopy: LM, SEM, TEM, confocal and applications --
Bioseparation techniques and their applications --
Preparation of monoclonal and polyclonal antibodies against specific protein(s).
Other Titles: Molecular and cellular methods in biology and medicine
Responsibility: Peter B. Kaufman [and others].

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