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Hedgehog signaling protocols

Author: Jamila I Horabin
Publisher: Totowa, N.J. : Humana Press, ©2007.
Series: Methods in molecular biology (Clifton, N.J.), v. 397.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the€pathway, which makes€it an incredibly important system to understand. This volume€examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses  Read more...
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Genre/Form: Electronic books
Laboratory Manuals
Additional Physical Format: Print version:
Hedgehog signaling protocols.
Totowa, N.J. : Humana ; [Paisley : Quantum, distributor], ©2007
(DLC) 2007932488
(OCoLC)123798709
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Jamila I Horabin
ISBN: 9781597455169 1597455164 1603271236 9781603271233
OCLC Number: 232302207
Description: 1 online resource (xvii, 256 pages) : illustrations.
Contents: Purifying the hedgehog protein and its variants / Darren P. Baker, Frederick R. Taylor, and R. Blake Pepinsky --
Application of sonic hedgehog to the developing chick limb / Eva Tieck and Cheryll Tickle --
Manipulation of hedgehog signaling in Xenopus by means of embryo microinjection and application of chemical inhibitors / Thomas Hollemann . [and others] --
Isolation of rat telencephalic neural explants to assay Shh GABAergic interneuron differentiation-inducing activity / Rina Mady and Jhumku D. Kohtz --
Genetic analysis of the vertebrate hedgehog-signaling pathway using muscle cell fate specification in the zebrafish embryo / Sudipto Roy --
Efficient manipulation of hedgehog/GLI signaling using retroviral expression systems / Maria Kasper [and others] --
Cell surface marker and cell cycle analysis, hedgehog signaling, and flow cytometry / Krsting Detmer and Ronald E. Garner --
Detecting tagged hedgehog with intracellular and extracellular immunocytochemistry for functional analysis / Ainhoa Callejo, Luis Quijada, and Isabel Guerrero --
Confocal analysis of hedgehog morphogenetic gradient coupled with fluorescent in situ hybridization of hedgehog target genes / Armel Gallet and Pascal P. Thérond --
RNA in the hedgehog signaling pathway : pFRiPE, a vector for temporally and spatially controlled RNAi in Drosophila / Eric Marois and Suzanne Eaton --
Germline clone analysis for maternally acting Drosophila hedgehog components / Erica M. Selva and Beth E. Stronach --
Clone analysis of hedgehog signaling in Drosophila somatic tissues / Christing M. Bankers and Joan E. Hooper --
GAL4/UAS targeted gene expression for studying Drosophila hedgehog signaling / Denise Bussona nd Anne-Marie Pret --
Biochemical fractionation of Drosophila cells / Melanie Stegman and David Robbins --
Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway / Chao Ton and Jin Jiang --
Sequence analyses to study the evolutionary history and cis-regulatory elements of hedgehog genes / Ferenc Müller and Anne-Gaelle Borycki.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 397.
Responsibility: edited by Jamila I. Horabin.

Abstract:

Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental  Read more...

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