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Heterologous expression of membrane proteins : methods and protocols

Author: Isabelle Mus-Veteau
Publisher: New York : Humana Press, ©2010.
Series: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 601.
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This book provides an overview of the different heterologous expression systems available to produce various membrane proteins for structural analysis. Chapters examine membrane protein  Read more...

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Genre/Form: Aufsatzsammlung
Additional Physical Format: Heterologous expression of membrane proteins.
New York, N.Y. : Humana Press, ©2010
(OCoLC)503006798
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Isabelle Mus-Veteau
ISBN: 9781607613435 1607613433 9781607613442 1607613441
OCLC Number: 503631054
Description: xi, 272 pages : illustrations ; 27 cm.
Contents: Heterologous expression of membrane proteins for structural analysis / Isabell Mus-Veteau.--
Production of membrane proteins in Escherichia coli and Lactococcus lactis / Eric R. Geertsma, Bert Poolman.--
Mammalian membrane receptors expression as inclusion bodies in Escherichia coli / Bernard Mouillac, Jean-Louis Baneres.--
Expression of membrane proteins at the Escherichia coli Membrane for structural studies / Manuela Zoonens, Bruno Miroux.--
Membrane protein expression in Lactococcus lactis / Annie Frelet-Barrand, Sylvain Boutigny, Edmund R.S. Kunji, Norbert Rolland.--
Heterologous expression of human membrane receptors in yeast Sacchraomyces cerevisiae / Olivier Joubert, Rony Nehme, Michel bidet, Isabelle Mus-Veteau.--
Mammalian membrane protein expression in baculovirus-infected insect cells / Celine Trometer, Pierre Falson.--
Expression of membrane proteins in Drosophila melanogaster S2 cells: production and analysis of a EGFP-fused G protein-coupled receptor as a model / Karl Brillet, Carlos A. Pereira, Renaud Wagner.--
Membrane protein epression in the eyes of transgenic flies / Valerie Panneels, Irmgard Sinning.--
Expression of mammalian membrane proteins in mammalian cells using semliki forest virus vectors / Kenneth Lundstrom.--
Membrane protein expression in cell-free systems / Birgit Schneider, Friederike Jnge, Vladimir A. Shirokov, Florian Durst, Daniel Schwarz, Volker Dotsch, Frank Bernhard.--
Practical considerations of membrane protein instability during purification and crystallization / Christopher G. Tate.--
Membrane protein solubilization / Katia Duquesne, James N. Sturgis.--
Amphipols and fluorinated surfactants: tow alternatives to detergents for studying ertrand Arnou, Marie Jidenko, Estelle Marchal, Marc Le Maire, Christine Jaxel.--
Amphipols and fluorinated surfactants: two alternatives to detergents for studying membrane proteins in vitro / Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc Le Maire, Christine Jaxel.--
Heterologous expression and affinity purification of eukaryotic membrane proteins in view of functional and structural studies: the example of the sarcoplasmic reticulum Ca2+-ATPase / Delphine Cardi, Cedric Montigny, Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc Le Maire, Christine Jaxel.
Series Title: Springer protocols (Series); Methods in molecular biology (Clifton, N.J.), v. 601.
Responsibility: edited by Isabelle Mus-Veteau.

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From the reviews:"Well-illustrated book provides an overview of different heterologous expression systems available to produce membrane proteins for structural analysis to facilitate understanding of Read more...

 
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