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Détails
| Type d’ouvrage : | Ressource Internet |
|---|---|
| Format : | Livre, Ressource Internet |
| Tous les auteurs / collaborateurs : |
Sharon A Doyle |
| ISBN : | 9781588298799 1588298795 9781597451963 1597451967 |
| Numéro OCLC : | 241042860 |
| Description : | xii, 322 p. : ill. (some col.) ; 26 cm. |
| Contenu : | High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning / Paul G. Blommel ... [et al.] -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.] -- "System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.]. Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh -- PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood -- High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina -- High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.] -- High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee -- The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens -- High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi -- Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.] -- Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh -- High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery. |
| Titre de collection : | Methods in molecular biology (Clifton, N.J.), 498. |
| Responsabilité : | edited by Sharon A. Doyle. |
| Plus d’informations : |
Résumé :
Critiques
Synopsis de l’éditeur
From the reviews: "The book consists 20 chapters, which cover methods in protein expression and purification summarized by leading experts in this area from USA, UK, and Switzerland. ! Numerous pictures in the text, a references list at the end of each chapter, and a subject index help better understand the presented materials. The volume is true handbook for protein biochemists, molecular biologists, biotechnologists, and other researchers and technicians who are involved in the many applicable fields." (G. Wiederschain, Biochemistry (Moscow), Vol. 74 (8), 2009) "High Throughput Protein Expression and Purification: Methods and Protocols, edited by Sharon A. Doyle, is an interesting attempt showing what one currently has at one's disposal if one wishes to make a first step toward this aim. ! this book should be recommended especially to researchers who would like to organize a new laboratory focused on high throughput protein production, or to improve an existing one. It can also prove helpful to those who wish to optimize individual protein expression, isolation or purification." (Marek Figlerowicz, Acta Biochimica Polonica, Vol. 56 (4), December, 2009) Lire la suite...
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Ouvrages semblables
Sujets associés :(10)
- Proteins -- Separation -- Methodology.
- Proteins -- genetics.
- Proteins -- isolation & purification.
- Gene Expression.
- Proteomics -- methods.
- Protéines -- Séparation.
- Proteine.
- Genexpression.
- Trennverfahren.
- Reinigungsverfahren.
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de adamstjean mise à jour 2011-08-01