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High-throughput RNAi screening : methods and protocols

Author: David O Azorsa; Shilpi Arora
Publisher: New York : Humana Press, [2016]
Series: Springer Protocols; Methods in molecular biology (Clifton, N.J.), v. 1470.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries.
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Genre/Form: Electronic books
Laboratory manuals
Additional Physical Format: Print version:
High-throughput Rnai Screening.
Humana Pr Inc 2016
(OCoLC)946907689
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: David O Azorsa; Shilpi Arora
ISBN: 9781493963379 1493963376
OCLC Number: 957740693
Description: 1 online resource (xi, 262 pages) : illustrations (some color)
Contents: Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening --
Optimization of Transfection Conditions for siRNA Screening --
High Throughput siRNA Screening Using Reverse Transfection --
Genome-Wide siRNA Screening Using Forward Transfection: Identification of Modulators of Membrane Trafficking in Mammalian Cells --
Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform --
A Protocol for a High-Throughput Multiplex Cell Viability Assay --
RNAi Screening of Leukemia Cells Using Electroporation --
Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening --
In Vitro Pooled shRNA Screening to Identify Determinants of Radiosensitivity --
Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens --
In Vitro High Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development --
High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans --
Design and Methods of Large-Scale RNA Interference Screens in Drosophila --
Genome-Wide RNAi Screening in C. elegans for Aging and Innate Immunity --
RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae --
RNAi Screening in Spodoptera frugiperda --
Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos --
Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens --
Data Analysis For High Throughput RNAi Screening. .
Series Title: Springer Protocols; Methods in molecular biology (Clifton, N.J.), v. 1470.
Responsibility: editors David O. Azorsa, Institute of Molecular Medicine, Phoenix Children’s Hospital, Phoenix, Arizona, USA; Shilpi Arora, Constellation Pharmaceuticals, Cambridge, Massachusetts, USA .

Abstract:

High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for  Read more...

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