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Host-fungus interactions : methods and protocols

Author: Alexandra C Brand; Donna M MacCallum
Publisher: New York : Humana Press : Springer, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 845.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This exploration of the culture and genetic manipulation of primary fungal pathogens, as well as interactions in model systems, includes detailed, lab-ready techniques as well as the key tips and  Read more...

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Genre/Form: Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Alexandra C Brand; Donna M MacCallum
ISBN: 1617795380 9781617795381 1617795399 9781617795398
OCLC Number: 756595133
Description: xvi, 597 pages : illustrations (some color) ; 27 cm.
Contents: Gene deletion in Candida albicans wild-type strains using the SAT1-flipping strategy --
Mini-blaster-mediated targeted gene disruption and marker complementation in Candida albicans --
Rapid detection of aneuploidy following the generation of mutants in Candida albicans --
Agrobacterium-mediated insertional mutagenesis in histoplasma capsulatum --
Targeted gene disruption in cryptococcus neoformans using double-joint PCR with split dominant selectable markers --
Multiple gene deletion in cryptococcus neoformans using the cre-lox system --
Gene disruption in aspergillus fumigatus using a PCR-based strategy and in vivo recombination in yeast --
Targeted gene deletion in aspergillus fumigatus using the hygromycin-resistance split-marker approach --
Gene disruption in coccidioides using hygromycin or phleomycin resistance markers --
RNAi-based gene silencing using a GFP sentinel system in histoplasma capsulatum --
RNA interference in cryptococcus neoformans --
Gene knockdown in paracoccidioides brasiliensis using antisense RNA --
Tetracycline-inducible gene expression in Candida albicans --
Galactose-inducible promoters in cryptococcus neoformans var. Grubii --
Modular gene over-expression strategies for Candida albicans --
Interactions between macrophages and cell wall oligosaccharides of Candida albicans --
Murine bone marrow-derived dendritic cells and t-cell activation by Candida albicans --
Phagocytosis and intracellular killing of Candida albicans by murine polymorphonuclear neutrophils --
Human oral keratinocytes: A model system to analyze host-pathogen interactions --
Simple assays for measuring innate interactions with fungi --
Binding and uptake of Candida albicans by human monocyte-derived dendritic cells --
Immune responses to Candida albicans in models of in vitro reconstituted human oral epithelium --
Analysis of host-cell responses by immunoblotting, ELISA, and real-time PCR --
In vitro model of invasive pulmonary aspergillosis in the human alveolus --
Biofilm formation studies in microtiter plate format --
Transcript profiling using ESTs from Paracoccidioides brasiliensis in models of infection --
Laser capture microdissection of Candida albicans from host tissue --
Isolation and amplification of fungal RNA for microarray analysis from host samples --
Cytokine measurement using cytometric bead arrays --
Transcript profiling of the murine immune response to invasive aspergillosis --
Caenorhabditis elegans: A nematode infection model for pathogenic fungi --
Drosophila melanogaster as a model organism for invasive aspergillosis --
Galleria mellonella as a model for fungal pathogenicity testing --
Embryonated chicken eggs as alternative infection model for pathogenic fungi --
Mouse intravenous challenge models and applications --
A nebulized intra-tracheal rat model of invasive pulmonary aspergillosis --
Invasive models of histoplasmosis --
Murine model of concurrent oral and vaginal Candida albicans colonisation --
A luciferase reporter for gene expression studies and dynamic imaging of superficial Candida albicans infections --
Modeling of fungal biofilms using a rat central vein catheter --
Orogastrointestinal model of mucosal and disseminated candidiasis --
A nonlethal murine cutaneous model of invasive aspergillosis.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 845.; Springer protocols (Series)
Responsibility: edited by Alexandra C. Brand, Donna M. MacCallum.

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From the book reviews:"The book `Host-fungus Interactions' assembles methods and protocols for studying gene disruption, modulation of gene expression, host and fungal responses to infection and Read more...

 
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