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Human cell culture protocols.

Author: Ragai R Mitry; Robin D Hughes
Publisher: New York : Humana Press/Springer, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), v. 806.; Springer protocols (Series)
Edition/Format:   Print book : English : 3rd ed.View all editions and formats
Database:WorldCat
Summary:

This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues.  Read more...

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Genre/Form: Laboratory Manuals
Laboratory manuals
Document Type: Book
All Authors / Contributors: Ragai R Mitry; Robin D Hughes
ISBN: 1617793663 9781617793660 1617793671 9781617793677
OCLC Number: 751802917
Description: xiv, 435 pages : illustrations (some color) ; 26 cm.
Contents: 1. Introduction to cell culture / Christina Philippeos, Robin D. Hughes, Anil Dhawan and Ragai R. Mitry --
2. Isolation and cultivation of dermal stem cells that differentiate into functional epidermal melanocytes / Ling Li, Mizubo Fukunaaga --
3. Isolation, cultivation, and application of human alveolar epithelial cells / Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, Hanno Huwer, and Claus-Michael Lehr --
4. Culture of parathyroid cells / Peyman Björklund and Per Hellman --
5. Functional analysis of human islets of langerhans maintained in culture / Shanta J. Persaud, Bo Liu, and Peter M. Jones --
6. Conversion of non-endocrine human pancreatic cells to insulin-producing cells for treatment of diabetes / Min Zhao and Guo Cai Huang --
7. Evaluation of cytochrome P450 activities in human hepatocytes in vitro / María José Gómez-Lechón, Agustín Lahoz, José V. Castell, and María Teresa Donato --
8. Human hepatocytes: isolation, culture, and quality procedures / Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, Peter Büchler, Michael Schoenberg, Jörg Kleeff, Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, Jan Hengstler, and Andrea K. Nussler --
9. Preclinical testing ov virotherapeutics for primary and secondary tumors of the liver / Martina Zimmermann, Timo Weiland, Michael Bitzer, and Ulrich M. Lauer --
10. Isolation and functional studies of human fetal gastric epithelium in primary culture / Pierre Chailler, Jean-Franc̦ois Beaulieu, and Daniel Ménard --
11. Isolation, characterization, and culture of normal human intestinal crypt and villus cells / Jean-Franc̦ois Beaulieu and Daniel Ḿenard --
12. Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts / Claire C. Sharpe and Mark E.C. Dockrell --
13. Glomerular epithelial and mesangial cell culture and characterization / Heather M. Wilson and Keith N. Stewart --
14. Culture of isolated human adipocytes and isolated adipose tissue / Kirstin A. Carwswell, Mi-Jeong Lee, and Susan K. Fried --
15. Primary culture of human adipocyte precursor cells: expansion and differentiation / Thomas Skurk and Hans Hauner --
16. Primary culture of ovarian cells for research on cell interactions in the hormonal control of steroidogenesis / Jerzy F. Galas --
17. Human vascular smooth muscle cell culture / Diane Proudfoot and Catherine Shanahan --
18. Culture of human endothelial cells from umbilical veins / Richard C.M. Siow --
19. Human peripheral blood mononuclear cell culture for flow cytometric analysis of phosphorylated mitogen-activated protein kinases / Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, and Dimitrios P. Bogdanos --
20. Human CD4+CD25(high [superscript]) CD127 (low/neg [superscript]) regulatory T cells / Haibin Su, Maria Serena Longhi, Pengyun Wang, Diego Vergani, and Yun Ma --
21. Human chondrocyte cultures as models of cartilage-specific gene regulation / Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring --
22. Isolation and culture of human osteoblasts / Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, and James A. Gallagher --
23. Human osteoclast culture and phenotypic characterization / Ankita Agrawal, James A. Gallagher, and Alison Gartland --
24. Effects of temperature generated from the holmium: Yag laser on human osteoblasts in monolayer tissue culture / Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, Ian D. McCarthy, and Al-Shymaa M. Hafez --
25. Laser microdissection microscopy: application to cell culture / Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia --
26. Automated adherent human cell culture (mesenchymal stem cells) / Robert Thomas and Elizabeth Ratcliffe --
27. Culturing and differentiating human mesenchymal stem cells for biocompatible scaffolds in regenerative medicine / William R. Otto and Catherine E. Sarraf.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 806.; Springer protocols (Series)
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