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Human embryonic stem cells handbook

Author: Kursad Turksen
Publisher: New York : Humana Press, ©2012.
Series: Methods in molecular biology (Clifton, N.J.), 873.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult. In the  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version:
Human embryonic stem cells handbook.
New York : Humana Press, ©2012
(DLC) 2012935581
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Kursad Turksen
ISBN: 9781617797941 1617797944 1617797936 9781617797934
OCLC Number: 792942623
Language Note: English.
Notes: Includes index.
Description: 1 online resource.
Contents: Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology --
Successful Scale-Up and Quality Assessments of Human Embryonic Stem Cells for Cell Therapy: Challenges and Overview --
Human Embryonic Stem Cells Derived in Xeno-Free Conditions --
Procedures for Derivation and Characterization of Human Embryonic Stem Cells from Odense, Denmark --
Principles for Derivation of Human Embryonic Stem Cells --
Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells --
Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos --
Analysis of LINE-1 Expression in Human Pluripotent Cells --
Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan --
Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos --
Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos --
Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders --
Single-Cell Enzymatic Dissociation of hESC Lines OxF1 --
OxF4 and Culture in Feeder-Free Conditions --
Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension --
Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells --
Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs) --
Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol --
Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells --
Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells --
Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next Generation Sequencing (MeDIP-seq) --
Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis.
Series Title: Methods in molecular biology (Clifton, N.J.), 873.
Responsibility: edited by Kursad Turksen.

Abstract:

Stem cell research has achieved much, yet still faces many challenges. In this comprehensive handbook, experts provide step-by-step, proven protocols allowing both novices and experienced researchers  Read more...

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