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Hydrocarbon and lipid microbiology protocols : field studies

Author: Terry J McGenity; K N Timmis; Balbina Nogales
Publisher: Berlin : Springer, ©2017.
Series: Springer protocols (Series)
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This Volume describes methods for investigating microbes in their natural environment and how to obtain representative samples and preserve them for subsequent analyses. Chapters are arranged according to the environments under investigation, which include: oil reservoirs, fracking fluids, aquifers, coal beds, oil sands and their tailing ponds, lakes, rivers, leaves, polar seas and ice, the sea-surface microlayer,  Read more...
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Genre/Form: Electronic books
Laboratory manuals
Laboratory Manuals
Additional Physical Format: Print version :
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Terry J McGenity; K N Timmis; Balbina Nogales
ISBN: 9783662531181 3662531186 366253116X 9783662531167
OCLC Number: 967230276
Description: 1 online resource (x, 362 pages) : illustrations (some color).
Contents: Introduction : field and in situ studies / Roger C. Prince --
Geological and hydrogeological characterization of subsurface / Daniel Hunkeler --
Sampling the subsurface / Thomas L. Kieft --
Protocols for investigating the microbial communities of oil and gas reservoirs / Nicolas Tsesmetzis, Michael J. Maguire, Ian M. Head, and Bart P. Lomans --
Protocols for investigating the microbiology of oil sands deposits / Christina M. Ridley, Gerrit Voordouw, and Lisa M. Gieg --
Protocols for investigating the microbiology of drilling fluids, hydraulic fracturing fluids, and formations in unconventional natural gas reservoirs / Christopher G. Struchtemeyer, Noha H. Youssef, and Mostafa S. Elshahed --
Protocols for investigating the microbiology of coal-bed-produced waters / Amy V. Callaghan and Boris Wawrik --
Protocols for handling, storing, and cultivating oil sands tailings ponds materials for microbial and molecular biological study / J.M. Foght, T. Siddique, and L.M. Gieg --
Strategies to map the microbiome of freshwater lakes : sampling and context / Stefan Bertilsson --
Collection and processing of river organisms and water column organisms / Sergi Sabater and Carles Borrego --
Protocols for the identification of phyllosphere-Induced Pollutant Degradation Genes / Tanja R. Scheublin --
Protocols for investigating hydrocarbon-oxidizing bacterial communities in polar seas and ice / Lo Giudice Angelina and Rizzo Carmen --
Sampling the sea surface microlayer / Michael Cunliffe and Oliver Wurl --
Protocols for radiotracer estimation of primary hydrocarbon oxidation in oxygenated seawater / Ryan Sibert, Sarah Harrison, and Samantha B. Joye --
Protocols for radiotracer estimation of methane oxidation rates at in situ methane concentrations in marine sediments / Ryan Sibert, Vladimir A. Samarkin, and Samantha B. Joye --
Protocols for mudflat and algal mat in situ analysis / Robert Duran and Cristiana Cravo-Laureau --
Protocols for the study of microbe-mineral interactions in modern microbialites / Estelle Couradeau, Karim Benzerara, David Moreira, and Purificacion Lopez-Garcıa --
Characterization of bacterial symbionts in deep-sea fauna : protocols for sample conditioning, fluorescence in situ hybridization, and image analysis / Sebastien Duperronv.
Series Title: Springer protocols (Series)
Responsibility: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors.

Abstract:

This Volume describes methods for investigating microbes in their natural environment and how to obtain representative samples and preserve them for subsequent analyses. Chapters are arranged according to the environments under investigation, which include: oil reservoirs, fracking fluids, aquifers, coal beds, oil sands and their tailing ponds, lakes, rivers, leaves, polar seas and ice, the sea-surface microlayer, mud flats, microbialites, and deep-sea fauna. A variety of downstream analytical procedures are described, including: nucleic-acid extraction and preparation for high-throughput sequencing, fluorescence in-situ hybridisation, and cultivation of aerobic and anaerobic hydrocarbon-degrading microbes. Though most chapters focus on hydrocarbon-rich environments, many of the approaches used are generic, and as such will be of value to researchers embarking on studies of microbes and their processes in the field. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

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