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Immuno-gold-labeling in cell biology

Author: A J Verkleij; J L M Leunissen
Publisher: Boca Raton, Fla. : CRC Press, ©1989.
Edition/Format:   Print book : EnglishView all editions and formats

Intends to describe and discuss the preparation of immuno-gold probes, the progress and development of preparation methods suitable for immuno-electron microscopy, and the use of immuno-gold labeling  Read more...


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Genre/Form: Aufsatzsammlung
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: A J Verkleij; J L M Leunissen
ISBN: 0849360536 9780849360534
OCLC Number: 18521378
Description: 364 pages : illustrations ; 26 cm
Contents: Preparation of Immuno-Gold Probes: Preparation of Gold Probes (J.L.M. Leunissen and J.R. de Mey). The Use of Colloidal Gold Particles for Testing the Specificity of Antibodies and/or Presence of Antigens (M. Moeremans, G. Daneels, M. de Raeymaeker, B. de Wever, and J.R. de Mey). Semi-Covalent Conjugation of Proteins to Gold-Dextran Particles (R.S> Molday and D.W. Laird). Quantitative Aspects of Labeling Colloidal Gold with Proteins (M. Horisberger). Electron Microscopic Methods for Immuno-Labeling: Cryosectioning According to Tokuyasu vs. Rapid Freezing, Freeze Substitution, and Resin Embedding (P. Sitte, K. Neumann, and L. Edelmann). Cryo-Ultramicrotomy and Immuno-Gold Labeling (J.L.M. Leunissen and A.J. Verkleij). Freeze Substitution for Immunochemistry (B. Humberl and H. Schwarz). Quantitative Assessment of Immuno-Gold Labeling in Cryosections (J.W.. Slot, G. Posthuma, L.Y. Chang, J.D. Crapo and H.J. Geuze). Replica Preparation Techniques in Immuno-Gold Cytochemistry (H. Hohenberg). Visual Thinking of Biological Membranes: From Freeze Etching to Label Fracture (P. Pinto da Silva). SEM-Immunocytochemistry with Small (5-15 nm) Colloidal Gold Markers (M. Muller, P. Walther, R. Hermann, and P. Schwarb). Detection and Use of Gold Probes with Video-Enhanced Contrast Light Microscope (M. de Brabander, H. Geerts, R. Nuydens, S. Geuens, M. Moeremans, and J.R. de Mey). Immuno-Gold Labeling and Confocal Scanning Laser Microscopy (W.A>M. Linnemans, G.J. Brakenhoff, E.A. van Spronsen, and N. Nanninga). Applications in Molecular Cell Biology: The Low Density Lipoprotein Receptor (A.M. Mommaas-Kienhuis and B.J. Vermeer). The Epidermal Growth Factor Receptor (J. Boonstra, N. van Belzen, P.M.P. van Bergen en Henegouwen, W.J. Hage, P. van maurik, F.A>C. Wiegant, and A.J. Verkleij). Immuno-Gold Labeling After Rapid Freezing Fixation and Freeze Substitution: Application to the Detection of Luciferase in Dinoflagellates (M.-T. Nicolas). Immuno-Gold Labeling of Escherichia coli Cell Envelope Components (W.F. Voorbout, J. Leunissen-Bijvelt, J.L.M. Leunissen, J. Tommassen, and A.J. Verkleij). Transport of Macromolecules through the Nuclear Pores (C.M. Feldherr and S.I. Dworetzky). Labeling of Viral Antigens in Replica Techniques (K. Mannweiler, W. Bohn, and G. Rutter). Detection in Cytoskeletal Proteins in Cultured Cells at the Ultrastructural Level (G. Langanger and J.R. de Mey). Index. Catalog no. 60531989, c. 368 pp., ISBN: 0-8493-6053-6U.S. $199.00/Outside U.S. $237.00
Responsibility: editors, A.J. Verkeleij, J.L.M. Leunissen.
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