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Immunoblotting.

Author: Kanopy (Firm)
Publisher: [San Francisco, California, USA] : Kanopy Streaming, 2015.
Edition/Format:   eVideo : Clipart/images/graphics
Summary:
This program explains the principles of immunoblotting with the aid of diagrams and demonstrates the technique in the laboratory. Immunoblotting explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself. The principles of immunoblotting are demonstrated and the transferring  Read more...
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Details

Material Type: Clipart/images/graphics, Internet resource, Videorecording
Document Type: Internet Resource, Computer File, Visual material
All Authors / Contributors: Kanopy (Firm)
OCLC Number: 921956015
Language Note: In English.
Notes: Title from title frames.
In Process Record.
Event notes: Originally produced by FENC in 2010.
Description: 1 online resource (streaming video file)
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Abstract:

This program explains the principles of immunoblotting with the aid of diagrams and demonstrates the technique in the laboratory. Immunoblotting explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bands over time and the fragility of the gel itself. The principles of immunoblotting are demonstrated and the transferring of information from the gel to a nitrocellulose membrane is described with the aid of diagrams. The techniques are then demonstrated in the laboratory by showing a method of making a single specific protein on the membrane visible using an antibody to that protein. The adding of a second antibody with an enzyme attached that will produce a coloured product when a substrate is added, is also shown.

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