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Immunochemistry in practice

Author: Alan Johnstone; Robin Thorpe
Publisher: Cambridge, Mass. : Blackwell Science, ©1996.
Edition/Format:   Print book : English : 3rd edView all editions and formats
Summary:
Immunochemical techniques are invaluable in all biological disciplines for detecting, estimating and isolating molecules and cells. The aim of this practical book is to provide scientists, whatever their specialty, with clear step-by-step details of immunochemical procedures. This third edition of Immunochemistry in Practice has been completely revised and updated; some chapters have been restructured and there are  Read more...
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Genre/Form: Laboratory Manuals
Document Type: Book
All Authors / Contributors: Alan Johnstone; Robin Thorpe
ISBN: 0865426333 9780865426337
OCLC Number: 33166514
Description: xv, 362 pages, [7] pages of plates : illustrations (some color) ; 25 cm
Contents: 1. Basic techniques --
2. Production of antibodies --
3. Purification of immunoglobulins, constituent chains and fragments --
4. Isolation and fractionation of lymphocytes --
5. Radiolabelling techniques --
6. Precipitation techniques in agar and agarose --
7. Analytical electrophoresis and isoelectric focusing --
8. Specific detection of antigens separated by polyacrylamide gel electrophoresis --
9. Preparative gel techniques --
10. Affinity chromatography and immunoprecipitation --
11. Coupling antibodies to fluorochromes, enzymes and biotin --
12. Immunoassays --
13. Immunocytochemistry, immunohistochemistry and flow cytofluorimetry --
Appendix 2: Buffers --
Appendix 3: Manufacturers and suppliers.
Responsibility: Alan Johnstone, Robin Thorpe.

Abstract:

This work provides scientists of all disciplines with clear descriptions of the wide range of immunochemical techniques available for detecting, isolating and characterizing biological molecules. It  Read more...

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