详细书目
| 材料类型: | 互联网资源 |
|---|---|
| 文件类型: | 书, 互联网资源 |
| 所有的著者/提供者: |
Werner Luttmann; et al |
| ISBN: | 0120885441 9780120885442 |
| OCLC号码: | 62381605 |
| 描述: | xiv, 243 p. : ill. ; 26 cm. |
| 内容: | 1. Antibodies. Antibody characteristics -- Fabrication of antibodies -- Purification of antibodies -- Chemical coupling and marking of antibodies -- 2. Cell separation. Separation by cell size and cell density: centrifugation techniques -- Separation by means of cell-specific surface molecules -- 3. Flow cytometry. How does that work? -- Fluorescences -- Sample preparation -- Start-up of the flow cytometer -- Compensation and measuring -- Analysis -- Models and configurations -- 4. Quantitative immunoassays. Assay concepts -- Radioimmunoassay (RIA) -- Enzyme-linked immunosorbent assay (ELISA) -- ELISPOT assay -- Particle immunoassay (PIA) -- Amplification systems. 5. Western blot. Sample preparation -- Protein separation by gel electrophoresis -- Transfer of proteins to a membrane (Blot) -- Protein detection -- Dot and slot blot -- 6. In situ immunolocalization. Analyses of cell suspensions -- Tissue analysis -- Immunoelectron microscopic tissue analysis -- 7. Immunoprecipitation. the classics -- Immunoprecipitation "today" -- 8. The cell: live, eat, die. Cell viability -- Cell proliferation -- Phagocytosis assays -- Cell-mediated cytotoxicity -- Apoptosis assays -- 9. Special immunoassays. Determination of blood groups -- HLA typing -- Lymphoblast transformation -- 10. A short excursion into the unbeloved world of statistics. Descriptive statistics -- Inferential statistics. |
| 丛书名: | Experimenter series. |
| 责任: | Werner Luttmann ... [et al.]. |
| 更多信息: |
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