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Immunology

著者: Werner Luttmann; et al
出版商: Amsterdam ; Boston : Elsevier Academic Press, ©2006.
丛书: Experimenter series.
版本/格式:   图书 : 英语查看所有的版本和格式
提要:

Providing the reader with tips and tricks for lab experiments, this work explores the methodological variety of immunology, and addresses the assets and drawbacks as well as critical points. It also  再读一些...

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材料类型: 互联网资源
文件类型: 书, 互联网资源
所有的著者/提供者: Werner Luttmann; et al
ISBN: 0120885441 9780120885442
OCLC号码: 62381605
描述: xiv, 243 p. : ill. ; 26 cm.
内容: 1. Antibodies. Antibody characteristics --
Fabrication of antibodies --
Purification of antibodies --
Chemical coupling and marking of antibodies --
2. Cell separation. Separation by cell size and cell density: centrifugation techniques --
Separation by means of cell-specific surface molecules --
3. Flow cytometry. How does that work? --
Fluorescences --
Sample preparation --
Start-up of the flow cytometer --
Compensation and measuring --
Analysis --
Models and configurations --
4. Quantitative immunoassays. Assay concepts --
Radioimmunoassay (RIA) --
Enzyme-linked immunosorbent assay (ELISA) --
ELISPOT assay --
Particle immunoassay (PIA) --
Amplification systems. 5. Western blot. Sample preparation --
Protein separation by gel electrophoresis --
Transfer of proteins to a membrane (Blot) --
Protein detection --
Dot and slot blot --
6. In situ immunolocalization. Analyses of cell suspensions --
Tissue analysis --
Immunoelectron microscopic tissue analysis --
7. Immunoprecipitation. the classics --
Immunoprecipitation "today" --
8. The cell: live, eat, die. Cell viability --
Cell proliferation --
Phagocytosis assays --
Cell-mediated cytotoxicity --
Apoptosis assays --
9. Special immunoassays. Determination of blood groups --
HLA typing --
Lymphoblast transformation --
10. A short excursion into the unbeloved world of statistics. Descriptive statistics --
Inferential statistics.
丛书名: Experimenter series.
责任: Werner Luttmann ... [et al.].
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