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In Situ Molecular Pathology and Co-Expression Analyses.

Author: Gerard J Nuovo
Publisher: Burlington : Elsevier Science, 2013.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
This new laboratory reference manual will explain, in easy-to-understand language, simplified ways to understand and perform in situ hybridization and immunohistochemistry tests. The book will also focus much attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Nuovo, Gerard J.
In Situ Molecular Pathology and Co-Expression Analyses.
Burlington : Elsevier Science, ©2013
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Gerard J Nuovo
ISBN: 9780124160019 0124160018
OCLC Number: 830163822
Notes: Step Two: Putting the Fixed Cells or Tissue on a Glass Slide.
Description: 1 online resource (298 pages)
Contents: Front Cover; In Situ Molecular Pathology and Co-Expression Analyses; Copyright Page; Contents; Acknowledgments; 1 Introduction; Suggested Readings; 2 The Basics of Molecular Pathology; Introduction; The Structure of DNA and RNA; The Basic Units of DNA and RNA; Homology; Key Terms in Molecular Biology; The Hydrogen Bond; The "Breathability" of the Hybridized Complexes; Denaturing and Annealing: Signal and Background; The Melting Temperature Curve; Probe Concentration and Probe Size. The Hybridization Signal with in Situ Hybridization is More Than the Melting Curve: The Effect of Target Copy NumberThe Concentration of Salt in the Stringent Wash; The Structure of Proteins; The Hybridization of a Primary Antibody and Its Epitope; Denaturing Protein-Protein Complexes; Detection Systems for Nucleic Acid-Nucleic Acid and Protein-Protein Hybrids; The Biochemical Basis of Signal versus Background; The Molecular-Based Assays; The Polymerase Chain Reaction; Southern Blot, Northern Blot, and Dot Blot Hybridization; Formalin Fixation and Molecular Hybridization; Immunohistochemistry. Suggested Readings3 The Biochemical Basis of In Situ Hybridization and Immunohistochemistry; Introduction; The Biochemical Effects of Fixing Cells in Formalin; Cross-linking of Protein Side Chains to Other Proteins, DNA, RNA, and Glycoproteins; Pore Size and Biochemical Charges; Relationship of Biochemical Changes to Fixation Time and Temperature; Relationship of Biochemical Changes to Cell Type; The Biochemical Effects of Embedding the Tissue/Cells into Paraffin; DNA and RNA Nicks from the Paraffin Embedding Process; Alterations in Proteins. The Biochemical Effects of Protease Digestion (Protease Pretreatment)Pore Size; Ionic Charge and Hydrogen Bond Potential of Cell/Breathability of the Macromolecules inside the Formalin-Fixed, Paraffin-Em ... ; The Practical Significance of the Highly Variable "Formalin-Induced THREE-DIMENSIONAL Network" on Optimizing the Signal for ... ; The Effect of Aging of the Tissue/Paraffin Block on the "Formalin-Induced THREE-DIMENSIONAL Network" for in Situ Hybridizat ... ; Molecular Basis of the Loss of Signal with in Situ Hybridization and Immunohistochemistry Relative to the Aging of the Slid ... "Recharging" the Aged Tissue to Allow for Enhanced in Situ Hybridization and ImmunohistochemistrySummary; Suggested Readings; 4 The Basics of In Situ Hybridization; Introduction; Step One: Fixing the Tissue or Cells; Buffered Formalin-Fixed Cells and Tissue; The Use of Fixatives Other Than Formalin: Cross-Linking Fixatives; The Use of Fixatives Other than Formalin: Denaturing Fixatives; Adding Some Additional Ingredients to Formalin; Unfixed Cells/Tissue; A Quick Review of the Consequences of Formalin Fixation in Performing in Situ Hybridization.

Abstract:

This new laboratory reference manual will explain, in easy-to-understand language, simplified ways to understand and perform in situ hybridization and immunohistochemistry tests. The book will also focus much attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. The automated platforms require that researchers and.

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