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  E-mail Message: I thought you might be interested in this item at http://www.worldcat.org/oclc/111601740 Title: Influence of temperature on Ranavirus infection in larval salamanders Ambystoma tigrinum. Publisher: ISBN/ISSN: 0177-5103 OCLC:111601740

  
  

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Influence of temperature on Ranavirus infection in larval salamanders Ambystoma tigrinum.

Author:	S Rojas; K Richards; JK Jancovich; EW Davidson
Edition/Format:	Article : English
Publication:	Diseases of aquatic organisms, 2005 Feb 28; 63(2-3): 95-100
Database:	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
Other Databases:	British Library Serials
Summary:	Temperature strongly influenced percent mortality and time to death of salamanders exposed to the Ambystoma tigrinum virus (iridovirus) (ATV). Most salamanders survived when exposed at 26 degrees C, whereas all died at 18 degrees C and nearly all died at 10 degrees C. Some asymptomatic salamanders that survived 60 d at 10 or 26 degrees C were found to be carrying virus. Polymerase chain reaction (PCR) confirmed the presence of virus in ATV-exposed salamanders but was found to be less sensitive than cell culture in detecting ATV at low concentrations. PCR products were 100% identical to ATV in the major capsid protein sequence. Virus titer was higher in salamanders held at 10 degrees C than at 18 degrees C but little virus, if any, was present in the small number of salamanders that died at 26 degrees C. These results may help explain periodic viral epizootics in field populations of A. tigrinum where water temperatures fluctuate widely.  Read more...
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