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Ion channels : methods and protocols

Author: Nikita Gamper
Publisher: New York : Humana Press : Springer, [2013] ©2013
Series: Methods in molecular biology (Clifton, N.J.), v. 998.; Springer protocols (Series)
Edition/Format:   Print book : English : Second editionView all editions and formats
Summary:
Every cell in our bodies contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signalling molecules. Maintenance and precise control of gating within these pathways are fundamental principles of life as these underlie basic cellular functions such as communication, contractility, and metabolism. In Ion Channels: Methods and Protocols,  Read more...
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Genre/Form: Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Nikita Gamper
ISBN: 9781627033503 1627033505
OCLC Number: 822974566
Notes: Previous edition: 2006.
Description: xiv, 440 pages : illustrations (some color) ; 26 cm.
Contents: 1. Approaches to cloning of pain-related ion channel genes / Armen N. Akopian --
2. Mammalian expression systems and transfection techniques / Daunia Laurenti and Lezanne Ooi --
3. Use of Escherichia coli for the production and purification of membrane proteins / Vincent G.L. Postis, Andrea E. Rawlings, Amelia Lesiuk, and Stephen A. Baldwin --
4. Transient overexpression of genes in neurons using nucleofection / Hannah M. Kirton, Louisa Pettinger, and Nikita Gamper --
5. Viral gene delivery : optimized protocol for production of high titer lentiviral vectors / James Hewinson, Julian F.R. Paton, and Sergey Kasparov --
6. Two-electrode voltage clamp / Bingcai Guan, Xingjuan Chen and Hailin Zhang --
7. Conventional micropipette-based patch clamp techniques /Jonathan D. Lippiat and David C. Wrighton --
8. Recording of ion channel activity in planar lipid bilayer experiments / Eleonora Zakharian --
9. Recording macroscopic currents in large patches from Xenopus oocytes / Tibor Rohacs --
10. Combined single-channel and macroscopic recording techniques to analyze gating mechanisms of the large conductance Ca² and voltage activated (BK) potassium channel / Nguyen V. Nguyen, Aleksandra Gruslova, Wojciech A. Kosiba and Bing Wang --
11. Perforated whole-cell patch-clamp recording / John E. Linley --
12. Piezo-electrically driven mechanical stimulation of sensory neurons / Jizhe Hao, Jérôme Ruel, Bertrand Coste, Yann Roudaut, Marcel Crest and Patrick Dlemas --
13. Automated planar patch-clamp / Carol J. Milligan and Clemens Möller --
14. Recording single-channel currents using "smart patch-clamp" technique / Anamika Bhargava and Julia Gorelik --
15. Using total internal reflection fluorescence microscopy to observe ion channel trafficking and assembly / Sarah Schwarzer, Gregory I. Mashanov, Justin E. Molloy, and Andrew Tinker --
16. Förster resonance energy transfer-based imaging at the cell surface of live cells / Sonya M. Bierbower and Mark S. Shapiro --
17. Use of dansyl-calmodulin to study interactions with channels and other proteins / Alessandro Alaimo, Covadonga Malo, Pilar Areso, Kerman Aloria, Oscar MIllet and Alvaro Villarroel --
18. Imaging and quantification of recycled K[subscript ATP] channels / Christopher J. Cockcroft --
19. Generation of antibodies that are externally acting isoform-specific inhibitors of ion channels / Jacqueline Naylor and David J. Beech --
20. Site-directed mutagenesis to study the structure-function relationships of ion channels / Wei Yang and Lin-Hua Jiang --
21. Cysteine-based cross-linking approach to study inter-domain interactions in ion channels / Lin-Hua Jiang --
22. Analysis of Ca²⁺⁻ binding sites in the MthK RCK domain by x-ray crystallography / Frank J. Smith and Brad S. Rothberg --
23. Isotope labeling strategies for analysis of an ion channel cytoplasmic domain by NMR spectroscopy / Karin Abarca-Heidemann, Elke Duchardt-Ferner, Jens Woehnert and Brad S. Rothberg --
24. Recording dendritic ion channel properties and function from cortical neurons / Mala M. Shah --
25. M-current recording from acute DRG slices / Kirstin E. Rose, Sylvain Gigout, and Nikita Gamper --
26. Studying ion channels in human erythrocytes by direct and indirect means / Stephan M. Huber --
27. Recording ion channels in isolated, split-opened tubules / Elena Mironova, Vladislav Bugay, Oleh Pochynyuk, Alexander Staruschenko and James D. Stockand --
28. Single-channel analysis of TRPC channels in the podocytes of freshly isolated glomeruli / Daria V. Ilatovskaya and Alexander Staruschenko --
29. Ca²+ imaging as a tool to assess TRP channel function in murine distal nephrons / Mykola Mamenko, Oleg Zaika, Roger G. O'Neil and Oleh Pochynyuk --
30. Patch-clamping Drosophila sensory neurons / Volodymyr Kucher, Benjamin A. Eaton, James D. Stockand and Nina Boiko --
31. Production and validation of recombinant adeno-associated virus for channelrhodopsin expression in neurons / John Y. Lin --
32. Optical control of ligand-gated ion channels / Stephanie Szobota, Catherine McKenzie, and Harald Janovjak.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 998.; Springer protocols (Series)
Responsibility: edited by Nikita Gamper, School of Biomedical Sciences, University of Leeds, UK.

Abstract:

This Methods in Molecular Biology (TM) book offers strategies and protocols for studying a large group of proteins that form ionic channels in the plasma membrane and intracellular membranes of  Read more...

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