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Ion-selective microelectrodes : principles, design, and application

Author: Daniel Ammann
Publisher: Berlin ; New York : Springer-Verlag, ©1986.
Series: Advances in Exprerimental Medicine and Biology, 50.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
The microelectrode technique is today the most widely used method in electrophy siology. Microelectrodes offer a unique approach to measurements of electrical pa rameters and ion activities of single cells. Several important breakthroughs in trans port physiology have arisen from microelectrode studies. Undoubtedly, there is a progressively wide-spread use of conventional and ion-selective microelectrodes. Due to  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Ammann, Daniel, 1947-
Ion-selective microelectrodes.
Berlin ; New York : Springer-Verlag, ©1986
(DLC) 85030423
(OCoLC)13002648
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Daniel Ammann
ISBN: 9783642525070 3642525075 9783540162223 3540162224
OCLC Number: 680192385
Reproduction Notes: Electronic reproduction. [S.l.] : HathiTrust Digital Library, 2010. MiAaHDL
Description: 1 online resource (xv, 346 pages) : illustrations.
Details: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Contents: Classification of Ion-Selective Electrodes --
Natural and Synthetic Neutral Carriers for Membrane Electrodes --
Liquid Membrane Electrodes Based on Neutral Carriers --
Potentiometric Measurements of Ion Activities with Neutral Carrier-Based Electrodes --
Construction of Liquid Membrane Microelectrodes --
Use of Neutral Carrier-Based Liquid Membrane Microelectrodes --
General Aspects of Intracellular Measurements of Ions --
Neutral Carrier-Based Liquid Membrane Microelectrodes --
Impact of Neutral Carrier Microelectrodes --
References.
Series Title: Advances in Exprerimental Medicine and Biology, 50.
Responsibility: Daniel Ammann.

Abstract:

The microelectrode technique is today the most widely used method in electrophy siology. Microelectrodes offer a unique approach to measurements of electrical pa rameters and ion activities of single cells. Several important breakthroughs in trans port physiology have arisen from microelectrode studies. Undoubtedly, there is a progressively wide-spread use of conventional and ion-selective microelectrodes. Due to their particular dimension and properties micro electrodes are exclusive ly applied to measurements on living matter. This must have many consequences to my thoughts on experiments with microelectrodes. In this book, my concern is fo cussing on the description of an intracellular method that should lead to reliable in formation on cellular parameters. The methodical basis for any meaningful applica tion is treated extensively. However, technical perfection and accurate results are not the only concern when working on animals and human beings. Rather, my thoughts are governed by the intellectual and moral mastery of the experimental ap proach on living subjects. A measurement with microelectrodes usually necessitates the sacrifice of an ani mal. This is an immense fact, and means that the knowledge gained by the experi ment must justify the death of a living subject.

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