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Isotope labeling in biomolecular NMR

Verfasser/in: Hanudatta S Atreya
Verlag: Dordrecht ; New York : Springer, ©2012.
Serien: Advances in experimental medicine and biology, v. 992.
Ausgabe/Format   E-Book : Dokument : EnglischAlle Ausgaben und Formate anzeigen
Datenbank:WorldCat
Zusammenfassung:
NMR spectroscopy has undergone a revolution in recent years with the advent of several new methods overcoming the problems of sensitivity and resolution. Recent developments in biotechnology have made it easier and economical to introduce 13C, 15N and 2H into proteins and nucleic acids. At the same time, there has been an explosion in the number of NMR experiments that utilize such isotope labeled samples. Thus, a  Weiterlesen…
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Gattung/Form: Electronic books
Medientyp: Dokument, Internetquelle
Dokumenttyp: Internet-Ressource, Computer-Datei
Alle Autoren: Hanudatta S Atreya
ISBN: 9789400749542 9400749546 9789400749535 9400749538
OCLC-Nummer: 817799578
Anmerkungen: Includes index.
Beschreibung: 1 online resource.
Inhalt: PROTEINS: Isotope Labeling Methods for Large Systems / Patrik Lundström, Alexandra Ahlner, Annica Theresia Blissing --
Segmental Labeling to Study Multidomain Proteins / Jing Xue, David S. Burz, Alexander Shekhtman --
Isotope Labeling for Solution and Solid-State NMR Spectroscopy of Membrane Proteins / Raffaello Verardi, Nathaniel J. Traaseth, Larry R. Masterson, Vitaly V. Vostrikov --
Isotope Labeling Methods for Relaxation Measurements / Patrik Lundström, Alexandra Ahlner, Annica Theresia Blissing --
Stereo-Array Isotope Labeling Method for Studying Protein Structure and Dynamics / Yohei Miyanoiri, Mitsuhiro Takeda, Masatsune Kainosho --
Amino Acid Selective Labeling and Unlabeling for Protein Resonance Assignments / Garima Jaipuria, B. Krishnarjuna, Somnath Mondal, Abhinav Dubey, Hanudatta S. Atreya --
NUCLEIC ACIDS: Isotope Labeling and Segmental Labeling of Larger RNAs for NMR Structural Studies / Olivier Duss, Peter J. Lukavsky, Frédéric H.-T. Allain --
METABOLOMICS: Isotope Enhanced Approaches in Metabolomics / G.A. Nagana Gowda, Narasimhamurthy Shanaiah, Daniel Raftery --
EXPRESSION SYSTEMS: Cell-Free Protein Synthesis Using E. coli Cell Extract for NMR Studies / Mitsuhiro Takeda, Masatsune Kainosho --
Isotope Labeling in Insect Cells / Alvar D. Gossert, Wolfgang Jahnke --
Mammalian Expression of Isotopically Labeled Proteins for NMR Spectroscopy / Mallika Sastry, Carole A. Bewley, Peter D. Kwong.
Serientitel: Advances in experimental medicine and biology, v. 992.
Verfasserangabe: Hanudatta S. Atreya, editor.

Abstract:

NMR spectroscopy has undergone a revolution in recent years with the advent of several new methods overcoming the problems of sensitivity and resolution. Recent developments in biotechnology have made it easier and economical to introduce 13C, 15N and 2H into proteins and nucleic acids. At the same time, there has been an explosion in the number of NMR experiments that utilize such isotope labeled samples. Thus, a combination of isotopic labeling and multidimensional, multinuclear NMR has opened up new avenues for structural studies of proteins, nucleic acids and their complexes. This book wil.

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