skip to content
JAK-STAT signalling : methods and protocols Preview this item
ClosePreview this item
Checking...

JAK-STAT signalling : methods and protocols

Author: Sandra E Nicholson; Nicos Nicola
Publisher: New York : Humana Press, ©2013.
Series: Methods in molecular biology (Clifton, N.J.), 967.; Springer protocols (Series)
Edition/Format:   Print book : EnglishView all editions and formats
Summary:

This Methods in Molecular Biology (TM) volume covers JAK and STAT specific approaches, the negative regulators of the pathway, and production and crystallization of JAK and STAT proteins, and more.  Read more...

Rating:

(not yet rated) 0 with reviews - Be the first.

Subjects
More like this

Find a copy online

Links to this item

Find a copy in the library

&AllPage.SpinnerRetrieving; Finding libraries that hold this item...

Details

Genre/Form: Laboratory manuals
Laboratory Manuals
Material Type: Internet resource
Document Type: Book, Internet Resource
All Authors / Contributors: Sandra E Nicholson; Nicos Nicola
ISBN: 9781627032414 162703241X 9781627032421 1627032428
OCLC Number: 805045150
Description: xi, 320 pages : illustrations ; 26 cm.
Contents: Analysis of Janus tyrosine kinase phosphorylation and activation / Jeremy A. Ross, Georgialina Rodriguez, and Robert A. Kirken --
Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins / Claude Haan and Serge Haan --
In vitro JAK kinase activity and inhibition assays / Jeffrey J. Babon and James M. Murphy --
Quantitative analysis of JAK binding using isothermal titration calorimetry and surface plasmon resonance / Jeffrey J. Babon --
Determination of protein turnover rates in the JAK/STAT pathway using a radioactive pulse-chase approach / Anna Dittrich, Elmar Siewert, and Fred Schaper --
Designing RNAi screens to identify JAK/STAT pathway components / Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler --
The use of JAK-specific inhibitors as chemical biology tools / Christopher J. Burns, David Segal, and Andrew F. Wilks --
Methods for detecting mutations in the human JAK2 gene / Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green --
Detection of activated STAT proteins / Jean-Patrick Parisien and Curt M. Horvath --
Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents / Serge Haan and Claude Haan --
Flow cytometric analysis of STAT phosphorylation / Jane Murphy and Gabrielle L. Goldberg --
Acetylation of endogenous STAT proteins / Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer --
Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining / Reza Khorooshi and Trevor Owens --
Nuclear trafficking of STAT proteins visualized by live cell imaging / Velasco Cimica and Nancy C. Reich --
Characterization of STAT self-association by analytical ultracentrifugation / Nikola Wenta and Uwe Vinkemeier --
Constitutively active STAT5 constructs / Lynn M. Heltemes-Harris and Michael A. Farrar --
Analysis of suppressor of cytokine signalling (SOCS) gene expression by real-time quantitative PCR / Tatiana B. Kolesnik and Sandra E. Nicholson --
Detection of endogenous SOCS1 and SOCS3 proteins by immunoprecipitation and western blot analysis / Jian-Guo Zhang and Sandra E. Nicholson --
In vitro ubiquitination and cytokine signaling components / Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw --
Production and crystallization of recombinant JAK proteins / Isabelle S. Lucet and Rebecca Bamert --
Bacterial expression, purification, and crystallization of tyrosine phosphorylated STAT proteins / Florence Baudin and Christoph W. Müller.
Series Title: Methods in molecular biology (Clifton, N.J.), 967.; Springer protocols (Series)
Responsibility: edited by Sandra E. Nicholson and Nicos A. Nicola.

Reviews

User-contributed reviews
Retrieving GoodReads reviews...
Retrieving DOGObooks reviews...

Tags

Be the first.
Confirm this request

You may have already requested this item. Please select Ok if you would like to proceed with this request anyway.

Linked Data


Primary Entity

<http://www.worldcat.org/oclc/805045150> # JAK-STAT signalling : methods and protocols
    a schema:CreativeWork, schema:Book ;
   library:oclcnum "805045150" ;
   library:placeOfPublication <http://id.loc.gov/vocabulary/countries/nyu> ;
   library:placeOfPublication <http://dbpedia.org/resource/New_York_City> ; # New York
   schema:about <http://experiment.worldcat.org/entity/work/data/1356320772#Topic/janus_kinases_metabolism> ; # Janus Kinases--metabolism
   schema:about <http://id.worldcat.org/fast/850288> ; # Cellular signal transduction
   schema:about <http://experiment.worldcat.org/entity/work/data/1356320772#Topic/stat_transcription_factors_metabolism> ; # STAT Transcription Factors--metabolism
   schema:about <http://id.loc.gov/authorities/subjects/sh85107660> ; # Protein kinases
   schema:about <http://id.loc.gov/authorities/subjects/sh85035236> ; # Cytokines
   schema:about <http://dewey.info/class/571.74/e23/> ;
   schema:about <http://id.worldcat.org/fast/1079695> ; # Protein kinases
   schema:about <http://id.loc.gov/authorities/subjects/sh89002397> ; # Cellular signal transduction
   schema:about <http://id.worldcat.org/fast/886268> ; # Cytokines
   schema:about <http://experiment.worldcat.org/entity/work/data/1356320772#Topic/signal_transduction> ; # Signal Transduction
   schema:bookFormat bgn:PrintBook ;
   schema:contributor <http://viaf.org/viaf/297710833> ; # Sandra E. Nicholson
   schema:contributor <http://viaf.org/viaf/109502084> ; # Nicos Nicola
   schema:copyrightYear "2013" ;
   schema:datePublished "2013" ;
   schema:description "Analysis of Janus tyrosine kinase phosphorylation and activation / Jeremy A. Ross, Georgialina Rodriguez, and Robert A. Kirken -- Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins / Claude Haan and Serge Haan -- In vitro JAK kinase activity and inhibition assays / Jeffrey J. Babon and James M. Murphy -- Quantitative analysis of JAK binding using isothermal titration calorimetry and surface plasmon resonance / Jeffrey J. Babon -- Determination of protein turnover rates in the JAK/STAT pathway using a radioactive pulse-chase approach / Anna Dittrich, Elmar Siewert, and Fred Schaper -- Designing RNAi screens to identify JAK/STAT pathway components / Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler -- The use of JAK-specific inhibitors as chemical biology tools / Christopher J. Burns, David Segal, and Andrew F. Wilks -- Methods for detecting mutations in the human JAK2 gene / Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green -- Detection of activated STAT proteins / Jean-Patrick Parisien and Curt M. Horvath -- Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents / Serge Haan and Claude Haan -- Flow cytometric analysis of STAT phosphorylation / Jane Murphy and Gabrielle L. Goldberg -- Acetylation of endogenous STAT proteins / Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer -- Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining / Reza Khorooshi and Trevor Owens -- Nuclear trafficking of STAT proteins visualized by live cell imaging / Velasco Cimica and Nancy C. Reich -- Characterization of STAT self-association by analytical ultracentrifugation / Nikola Wenta and Uwe Vinkemeier -- Constitutively active STAT5 constructs / Lynn M. Heltemes-Harris and Michael A. Farrar -- Analysis of suppressor of cytokine signalling (SOCS) gene expression by real-time quantitative PCR / Tatiana B. Kolesnik and Sandra E. Nicholson -- Detection of endogenous SOCS1 and SOCS3 proteins by immunoprecipitation and western blot analysis / Jian-Guo Zhang and Sandra E. Nicholson -- In vitro ubiquitination and cytokine signaling components / Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw -- Production and crystallization of recombinant JAK proteins / Isabelle S. Lucet and Rebecca Bamert -- Bacterial expression, purification, and crystallization of tyrosine phosphorylated STAT proteins / Florence Baudin and Christoph W. Müller."@en ;
   schema:exampleOfWork <http://worldcat.org/entity/work/id/1356320772> ;
   schema:genre "Laboratory manuals"@en ;
   schema:genre "Laboratory Manuals"@en ;
   schema:inLanguage "en" ;
   schema:isPartOf <http://worldcat.org/issn/1949-2448> ; # Springer protocols (Series)
   schema:isPartOf <http://worldcat.org/issn/1064-3745> ; # Methods in molecular biology (Clifton, N.J.) ;
   schema:isPartOf <http://experiment.worldcat.org/entity/work/data/1356320772#Series/springer_protocols> ; # Springer protocols
   schema:name "JAK-STAT signalling : methods and protocols"@en ;
   schema:productID "805045150" ;
   schema:publication <http://www.worldcat.org/title/-/oclc/805045150#PublicationEvent/new_york_humana_press_2013> ;
   schema:publisher <http://experiment.worldcat.org/entity/work/data/1356320772#Agent/humana_press> ; # Humana Press
   schema:url <http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-242-1> ;
   schema:workExample <http://worldcat.org/isbn/9781627032414> ;
   schema:workExample <http://worldcat.org/isbn/9781627032421> ;
   umbel:isLike <http://bnb.data.bl.uk/id/resource/GBB2A5545> ;
   wdrs:describedby <http://www.worldcat.org/title/-/oclc/805045150> ;
    .


Related Entities

<http://dbpedia.org/resource/New_York_City> # New York
    a schema:Place ;
   schema:name "New York" ;
    .

<http://experiment.worldcat.org/entity/work/data/1356320772#Series/springer_protocols> # Springer protocols
    a bgn:PublicationSeries ;
   schema:hasPart <http://www.worldcat.org/oclc/805045150> ; # JAK-STAT signalling : methods and protocols
   schema:name "Springer protocols" ;
    .

<http://experiment.worldcat.org/entity/work/data/1356320772#Topic/janus_kinases_metabolism> # Janus Kinases--metabolism
    a schema:Intangible ;
   schema:name "Janus Kinases--metabolism"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1356320772#Topic/signal_transduction> # Signal Transduction
    a schema:Intangible ;
   schema:name "Signal Transduction"@en ;
    .

<http://experiment.worldcat.org/entity/work/data/1356320772#Topic/stat_transcription_factors_metabolism> # STAT Transcription Factors--metabolism
    a schema:Intangible ;
   schema:name "STAT Transcription Factors--metabolism"@en ;
    .

<http://id.loc.gov/authorities/subjects/sh85035236> # Cytokines
    a schema:Intangible ;
   schema:name "Cytokines"@en ;
    .

<http://id.loc.gov/authorities/subjects/sh85107660> # Protein kinases
    a schema:Intangible ;
   schema:name "Protein kinases"@en ;
    .

<http://id.loc.gov/authorities/subjects/sh89002397> # Cellular signal transduction
    a schema:Intangible ;
   schema:name "Cellular signal transduction"@en ;
    .

<http://id.worldcat.org/fast/1079695> # Protein kinases
    a schema:Intangible ;
   schema:name "Protein kinases"@en ;
    .

<http://id.worldcat.org/fast/850288> # Cellular signal transduction
    a schema:Intangible ;
   schema:name "Cellular signal transduction"@en ;
    .

<http://id.worldcat.org/fast/886268> # Cytokines
    a schema:Intangible ;
   schema:name "Cytokines"@en ;
    .

<http://viaf.org/viaf/109502084> # Nicos Nicola
    a schema:Person ;
   schema:birthDate "1950" ;
   schema:familyName "Nicola" ;
   schema:givenName "Nicos" ;
   schema:name "Nicos Nicola" ;
    .

<http://viaf.org/viaf/297710833> # Sandra E. Nicholson
    a schema:Person ;
   schema:familyName "Nicholson" ;
   schema:givenName "Sandra E." ;
   schema:name "Sandra E. Nicholson" ;
    .

<http://worldcat.org/isbn/9781627032414>
    a schema:ProductModel ;
   schema:isbn "162703241X" ;
   schema:isbn "9781627032414" ;
    .

<http://worldcat.org/isbn/9781627032421>
    a schema:ProductModel ;
   schema:isbn "1627032428" ;
   schema:isbn "9781627032421" ;
    .

<http://worldcat.org/issn/1064-3745> # Methods in molecular biology (Clifton, N.J.) ;
    a bgn:PublicationSeries ;
   schema:hasPart <http://www.worldcat.org/oclc/805045150> ; # JAK-STAT signalling : methods and protocols
   schema:issn "1064-3745" ;
   schema:name "Methods in molecular biology (Clifton, N.J.) ;" ;
   schema:name "Methods in molecular biology," ;
    .

<http://worldcat.org/issn/1949-2448> # Springer protocols (Series)
    a bgn:PublicationSeries ;
   schema:hasPart <http://www.worldcat.org/oclc/805045150> ; # JAK-STAT signalling : methods and protocols
   schema:issn "1949-2448" ;
   schema:name "Springer protocols (Series)" ;
    .


Content-negotiable representations

Close Window

Please sign in to WorldCat 

Don't have an account? You can easily create a free account.