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Kidney research : experimental protocols

Author: Tim D Hewitson; Gavin J Becker
Publisher: New York, NY : Humana Press, ©2009.
Series: Methods in molecular biology (Clifton, N.J.), v. 466.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Database:WorldCat
Summary:
As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by  Read more...
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Genre/Form: Laboratory Manuals
Electronic books
Additional Physical Format: Print version:
Kidney research.
New York : Humana, ©2009
(DLC) 2008927373
(OCoLC)227033897
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Tim D Hewitson; Gavin J Becker
ISBN: 9781597453523 1597453528
OCLC Number: 311865091
Description: 1 online resource (xv, 268 pages) : illustrations (some color).
Contents: Isolation and propagation of glomerular mesangial cells / Paolo Mené and Antonella Stoppacciaro --
Isolation and primary culture of human proximal tubule cells / David A. Vesey [and others] --
Propagation and culture of renal fibroblasts / Lauren Grimwood and Rosemary Masterson --
Small animal models of kidney disease : a review / Tim D. Hewitson, Takahiko Ono, and Gavin J. Becker --
Measurement of glomerular filtration rate in conscious mice / Zhonghua Qi and Matthew D. Breyer --
Laser capture microdissection of kidney tissue / Robert P. Woroniecki and Erwin P. Bottinger --
Quantitative gene expression analysis in kidney tissues / Chris Tikellis [and others] --
In vivo imaging of leukocyte recruitment to glomeruli in mice using intravital microscopy / A. Richard Kitching, Michael P. Kuligowski, and Michael J. Hickey --
Using in situ hybridization to localize renal gene expression in tissue sections / Ian A. Darby, Alexis Desmoulière, and Tim D. Hewitson --
Immuno and lectin histochemistry for renal light microscopy / Tim D. Hewitson and Lauren Grimwood --
Immuno and lectin histochemistry for renal electron microscopy / Mitsuru Nakajima --
Pimonidazole adduct immunohistochemistry in the rat kidney : detection of tissue hypoxia / Christian Rosenberger [and others] --
Identification of apoptosis in kidney tissue sections / Glenda Gobe --
Cell-populated floating collagen lattices : an in vitro model of parenchymal contraction / Kristen J. Kelynack --
Mechanical stretch-induced signal transduction in cultured mesangial cells / Joan Krepinsky --
Determination of collagen content, concentration, and sub-types in kidney tissue / Chrishan S. Samuel --
SELDI-TOF mass spectrometry-based protein profiing of kidney tissue / Eleni Giannakis [and others] --
In vivo transfer of small interfering RNA or small hairpin RNA targeting glomeruli / Yoshitsugu Takabatake, Yoshitaka Isaka, and Enyu Imai.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 466.
Responsibility: edited by Tim D. Hewitson, Gavin J. Becker.

Abstract:

This cutting-edge book delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney. It includes step-by-step instructions providing a  Read more...

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From the reviews: "This book details research methodologies especially relevant for nephrological studies. ... Researchers in the field of nephrology are the intended audience. The book covers Read more...

 
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