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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

Author: Thomas Meier; F Fahrenholz
Publisher: Basel : Birkhäuser Basel, 1995.
Series: BioMethods.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Electronic books
Additional Physical Format: Printed edition:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Thomas Meier; F Fahrenholz
ISBN: 9783034873499 3034873492 9783034873512 3034873514
OCLC Number: 840290185
Description: 1 online resource.
Contents: 1 An Introduction to Avidin-Biotin Technology and Options for Biotinylation --
1.1 Introduction --
1.2 Technical Procedures --
1.3 Troubleshooting --
Acknowledgments --
References --
2 Synthesis of Photocleavable Biotinylated Ligands and Application for Affinity Chromatography --
Summary --
2.1 Introduction --
2.2 Technical Procedures --
2.3 Results and Discussion --
2.4 Troubleshooting --
References --
3 Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands --
Summary --
3.1 Introduction --
3.2 Technical Procedures --
3.3 Results and Discussion --
3.4 Troubleshooting --
Acknowledgments --
References --
4 Photoreactive Biotinylated Peptide Ligands for Affinity Labeling --
Summary --
4.1 Introduction --
4.2 Technical Procedures --
4.3 Results and Discussion --
4.4 Troubleshooting --
References --
5 Immunoprecipitation of Biotinylated Cell Surface Proteins --
Summary --
5.1 Introduction --
5.2 Technical Procedures --
5.3 Results and Discussion --
5.4 Troubleshooting --
Acknowledgments --
References --
6 Biotinylation and Chemical Cross-Linking of Membrane Associated Molecules --
Summary --
6.1 Introduction --
6.2 Technical Procedures --
6.3 Results and Discussion --
6.4 Troubleshooting --
Acknowledgments --
References --
7 Preparation of Biotinylated Lectins and Application in Microtiter Plate Assays and Western Blotting --
Summary --
7.1 Introduction --
7.2 Technical Procedures --
7.3 Results and Discussion --
7.4 Troubleshooting --
Acknowledgements --
References --
8 Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions --
Summary --
8.1 Introduction --
8.2 Technical Procedures --
8.3 Results and Discussion --
8.4 Troubleshooting --
Acknowledgments --
References --
9 Preparation and Use of Biotinylated Probes for the Detection and Characterisation of Serine Proteinase and Serine Proteinas Inhibitory Proteins --
Summary --
9.1 Introduction --
9.2 Technical Procedures --
9.3 Results and Discussion --
9.4 Troubleshooting --
Acknowledgments --
References --
10 Avidin/Biotin-Mediated Conjugation of Antibodies to Erythrocytes: An Approach for Immunoerythrocyte Exploration in vivo --
Summary --
10.1 Introduction --
10.2 Technical Procedures --
10.3 Results and Discussion --
10.4 Troubleshooting --
Acknowledgments --
References --
11 Biotin in vitro Translation: A Nonradioactive Method for the Synthesis of Biotin-Labeled Proteins in a Cell-Free System --
Summary --
11.1 Introduction --
11.2 Technical Procedures --
11.3 Results and Discussion --
11.4 Troubleshooting --
Acknowledgments --
References --
12 Nonradioactive Detection of Nucleic Acids with Biotinylated Probes --
Summary --
12.1 Introduction --
12.2 Technical procedures --
12.3 Results and discussion --
12.4 Troubleshooting --
References --
13 Biotin-Labeled Riboprobes to Study RNA-Binding Proteins --
Summary --
13.1 Introduction --
13.2 Technical Procedures --
13.3 Results and Discussion --
13.4 Troubleshooting --
Acknowledgments --
References --
14 Rapid YAC End Sequencing by Alu-Vector PCR and Biotinylated Primers --
Summary --
14.1 Introduction --
14.2 Technical Procedures --
14.3 Results and Discussion --
14.4 Troubleshooting --
Acknowledgments --
References.
Series Title: BioMethods.
Responsibility: edited by Thomas Meier, Falk Fahrenholz.

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